LEU-RO

Leupeptina

powder, from synthetic, =96.5%

• Sinonimo/i: Acetil-Leu-Leu-Arg-al, N-acetil-L-leucilL-leucil-L-arginina
• Formula condensata: C₂₀H₃₈N₆O₄ · 1/2H₂SO₄
• Numero CAS: 103476-89-7
• Peso molecolare: 475.59
• Numero MDL: MFCD00037012
• Codice UNSPSC: 12352202
• ID PubChem: 329817679

Proprietà

• Origine biologica: synthetic
• Livello qualitativo: 100
• Descrizione: Ac-Leu-Leu-argininal x 1/2 H₂SO₄, synthetic
• Saggio: 96.5%
• Forma fisica: powder
• PM: M_r 475.6
• Confezionamento: pkg of 100 mg (11529048001); pkg of 25 mg (11017128001); pkg of 5 mg (11017101001); pkg of 50 mg (11034626001)
• Produttore/marchio commerciale: Roche
• Solubilità: H₂O: 50 mg/mL
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: OS(O)(=O)=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O
• InChI: 1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1; CIPMKIHUGVGQTG-VFFZMTJFSA-N

Descrizione

Specificità

Leupeptin inhibits serine and thiol proteases such as trypsin, plasmin, proteinase K, kallikrein, papain, thrombin, and cathepsin A and B.
Not affected are α-, β-, γ- and δ- chymotrypsin, pepsin, cathepsin D, elastase, renin, and thermolysin.

Applicazioni

Leupeptin as well as other protease inhibitors like antipain, chymostatin, pepstatin, and phosphoramidon are useful for the protection of proteins during their isolation from tissues or membranes. Leupeptin can be removed from the reaction by dialysis.
Note: To check other protease inhibitors, try our Protease Inhibitor Set including Antipain Dihydrochloride, Aprotinin, Bestatin, Chymostatin, E-64, EDTA-Na₂, Leupeptin, Pefabloc SC, Pepstatin, and Phosphoramidon.

Azioni biochim/fisiol

Inibitore delle serina e cisteina proteasi. Inibisce plasmina, tripsina, papaina, calpaina e catepsina B. Non inibisce pepsina, catepsina A e D, trombina o α-chimotripsina. Concentrazione efficace 10-100 μM. Sono stati effettuati numerosi studi con la leupeptina per proteggere dalla perdita dell′udito causata dalla iperstimolazione acustica o dall′antibiotico ototossico gentamicina. (Si ritiene che la perdita delle cellule ciliate della coclea sia mediata dalla calpaina.)

Caratteristiche e vantaggi

Contents
Synthetic, white powder

Qualità

Function test: Performance controlled with trypsin.

Nota sulla preparazione

Working concentration: 0.5 to 5 μg/ml (1 to 20 μM)

Comparison of working concentrations of pefabloc with leupeptin and PMSF is in files.

Working solution: Solvent is recommended in distilled water.
Highly soluble in water (1 mg/ml), methanol, ethanol, acetic acid, dimethyl formamide and dimethyl sulfoxide.
Poorly soluble in acetone, chloroform, ethyl ether and n-hexane.

Storage conditions (working solution): -15 to -25 °C
In aqueous solution leupeptin is stable for 1 month at 2 to 8 °C or for at least 6 months at - 15 to -25 °C, stored under nitrogen. For best results, freeze the dissolved inhibitor in aliquots and avoid repeated thawing.

Ricostituzione

Highly soluble in water (1 mg/ml). Stable for at least one week at 2 to 8 °C and 6 months frozen in aliquots at -15 to -25 °C.

Risultati analitici

Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302 + H312 + H332
• Consigli di prudenza: P261 - P264 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P501
• Classi di pericolo: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable