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11633716001

Roche

Anti-Digoxigenin-POD (poly), Fab fragments

from sheep

Sinonimo/i:

anti-digoxigenin, digoxigenin

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About This Item

Codice UNSPSC:
12352203

Origine biologica

sheep

Livello qualitativo

100

Coniugato

peroxidase conjugate

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

lyophilized

Confezionamento

pkg of 50 U

Produttore/marchio commerciale

Roche

Isotipo

IgG

Temperatura di conservazione

2-8°C

Descrizione generale

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.

Specificità

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Applicazioni

Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:
  • whole-mount in situ hybridization
  • fluorescence in situ hybridization
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • Southern blot
  • western blot
  • double labeling experiments
  • lectin blots

Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.

Nota sulla preparazione

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • Dot blot: 50 to 100 mU/ml
  • ELISA: 2 to 50 mU/ml
  • Western blot: 50 to 100 mU/ml
  • Southern blot: 50 to 100 mU/ml

Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Ricostituzione

Add 1 ml double-distilled water to a final concentration of 50 U/ml.

Altre note

For life science research only. Not for use in diagnostic procedures.

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Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H317

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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