10108057001

Pepsin

lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

• Sinonimo/i: pepsin
• Classificazione EC (Enzyme Commission): 3.4.23.1 (BRENDA, IUBMB)
• Codice UNSPSC: 12352204

Proprietà

• Origine biologica: pig gastric mucosa
• Livello qualitativo: 100
• Forma fisica: lyophilized (salt-free)
• Attività specifica: ~2500 units/mg protein
• Confezionamento: pkg of 1 g
• Produttore/marchio commerciale: Roche
• pH ottimale: 1.8-2.2
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Pepsin is an aspartic endoproteinase isolated from pig gastric mucosa. It is mainly used for the unspecific hydrolysis of proteins and peptides in acidic media. It also provides a limited hydrolysis of native immunoglobulins to yield biologically active fragments.

Specificità

Aspartic endopeptidase with relatively broad specificity. Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1′ positions.

Applicazioni

Pepsin has been used for the digestion of liver tissue obtained from rat.

Nota sulla preparazione

Working concentration: 0.05 to 0.5 mg/ml
Storage conditions (working solution): A pepsin solution is stable at 2 to 8 °C, at least for one week, at neutral pH and under germfree conditions. Frozen aliquots of the enzyme solution are expected to be more stable.

A stock solution in 10 mM HCl (5-10 mg/ml) should be stable for at least one week at 2 to 8 °C, too. Storage light protected, tightly closed.

A short (2 minutes) incubation at 40 °C has negligible effect on the activity of lyophilized pepsin or pepsin in solution.

Risultati analitici

At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Pittogrammi: GHS07,GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H319,H334,H335
• Consigli di prudenza: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Classi di pericolo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable