MABT398
Anti-CD226/DNAM-1 Antibody, clone LeoA1 (Azide Free)
clone LeoA1, from mouse
Sinonimo/i:
CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD_antigen=CD226
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
LeoA1, monoclonal
Reattività contro le specie
human
tecniche
activity assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable
Isotipo
IgG1κ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... CD226(10666)
Descrizione generale
CD226/DNAM1 is a T cell expressed plasma membrane receptor protein that plays multiple roles in lymphocyte signaling, intercellular adhesion, lymphokine secretion and cytotoxic T-cell and NK cell mediated killing. CD226 activates natural killer (NK) cell-mediated cytotoxicity and is a key player in the immune synapse between killer T cells and targets. Also, because it recognizes CD155 and nectins CD226/DNAM1 is being examined as a potential anti-cancer agent because soluble CD226 directly inhibits tumor growth by binding CD155 expressed on tumor cells and down regulating proliferation. CD226/DNAM-1 is expressed on Th1, but not Th2 or Th0 cells, it is also expressed on NK cells, CD8+ T-cells, but not developing T cells. CD226/DNAM-1 is also expressed on monocytes and platelets.
Specificità
MABT398 does not work by western blotting under reducing conditions (for this application please see MABT341).
Immunogeno
Whole cells corresponding to human CD226/DNAM-1.
Applicazioni
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CD226/DNAM-1 in CD226 transfected CHO cells and non-transfected CHO cells.
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected CD226/DNAM-1 in 1X10E6 CD226 transfected CHO cells.
Activity Assay Analysis: A representative lot detected CD226/DNAM-1 which induces platelet aggregation (Scott, J.L., et al. (1989). Journal of Biol. Chem. 264(23):13475-13482).
Competative Binding Assay Analysis: A representative lot detected CD226/DNAM-1 in the binding of this antibody to resting and pharmacologically activated PHA-induced blasts and Jurkat cells (Sherrington, P.D., et al. (1997). Journal of Biol. Chem. 272:21735-21744).
Inhibition Assay Analysis: A representative lot detected CD226/DNAM-1 which inhibited the induction of both CTL andAK cells from their precursors (Burns, G.F., et al. (1985). J. Exp. Med. 161:1063-1078).
Immunoprecipitation Analysis: A representative lot detected CD226/DNAM-1 in MLA-I44 cells (Burns, G.F., et al. (1985). J. Exp. Med. 161:1063-1078).
Immunoprecipitation Analysis: A representative lot detected CD226/DNAM-1 in Jurkat PTA-1 transfectants stimulated with TPA (Ralston, K.J., et al. (2004). Journal of Biol. Chem. 279:33816-33828).
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected CD226/DNAM-1 in 1X10E6 CD226 transfected CHO cells.
Activity Assay Analysis: A representative lot detected CD226/DNAM-1 which induces platelet aggregation (Scott, J.L., et al. (1989). Journal of Biol. Chem. 264(23):13475-13482).
Competative Binding Assay Analysis: A representative lot detected CD226/DNAM-1 in the binding of this antibody to resting and pharmacologically activated PHA-induced blasts and Jurkat cells (Sherrington, P.D., et al. (1997). Journal of Biol. Chem. 272:21735-21744).
Inhibition Assay Analysis: A representative lot detected CD226/DNAM-1 which inhibited the induction of both CTL andAK cells from their precursors (Burns, G.F., et al. (1985). J. Exp. Med. 161:1063-1078).
Immunoprecipitation Analysis: A representative lot detected CD226/DNAM-1 in MLA-I44 cells (Burns, G.F., et al. (1985). J. Exp. Med. 161:1063-1078).
Immunoprecipitation Analysis: A representative lot detected CD226/DNAM-1 in Jurkat PTA-1 transfectants stimulated with TPA (Ralston, K.J., et al. (2004). Journal of Biol. Chem. 279:33816-33828).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This Anti-CD226/DNAM-1 Antibody, clone LeoA1 (Azide Free) is validated for use in Western Blotting and Immunocytochemistry and Flow Cytometry and Immunoprecipitation and Inhibition and Activity Assay for the detection of CD226/DNAM-1.
Qualità
Evaluated by Western Blotting in CD226 transfected CHO cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD226/DNAM-1 in 10 µg of CD226 transfected CHO cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD226/DNAM-1 in 10 µg of CD226 transfected CHO cell lysate.
Descrizione del bersaglio
~39 kDa observed
Stato fisico
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Stoccaggio e stabilità
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Altre note
Concentration: Please refer to lot specific datasheet.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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G F Burns et al.
The Journal of experimental medicine, 161(5), 1063-1078 (1985-05-01)
The characteristics of a novel T lineage-specific activation antigen, termed TLiSA1, are described. The antigen was detected with a mouse monoclonal antibody, LeoA1, that was raised against activated human T cells generated in mixed lymphocyte culture (MLC). The antigen became
J L Scott et al.
The Journal of biological chemistry, 264(23), 13475-13482 (1989-08-15)
When platelets bind certain specific ligands they are induced to secrete the contents of their cytoplasmic granules and to aggregate. Studies of the molecular events accompanying this vital physiological response have led to a greater understanding of cell activation in
P D Sherrington et al.
The Journal of biological chemistry, 272(35), 21735-21744 (1997-08-29)
T lineage-specific activation antigen 1 (TLiSA1) antigen was initially described as a T lineage-specific activation antigen involved in the differentiation of human cytotoxic T cells. Subsequently, the antigen was identified on platelets and was shown to be involved in platelet
Kylie J Ralston et al.
The Journal of biological chemistry, 279(32), 33816-33828 (2004-05-13)
Clustering of the T cell integrin, LFA-1, at specialized regions of intercellular contact initiates integrin-mediated adhesion and downstream signaling, events that are necessary for a successful immunological response. But how clustering is achieved and sustained is not known. Here we
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