MABN842

Anti-PERK Antibody, clone 4F6.1

clone 4F6.1, from mouse

• Sinonimo/i: PERK, Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 4F6.1, monoclonal
• Reattività contro le specie: human
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_004827
• N° accesso UniProt: Q9NZJ5
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... EIF2AK3(9451)

Descrizione

Descrizione generale

Eukaryotic translation initiation factor 2-alpha kinase 3 (EC 2.7.11.1; UniProt Q9NZJ5; also known as Eukaryotic translation initiation factor 2 alpha kinase 3, HsPEK, Pancreatic EIF2-alpha kinase, PRKR-like endoplasmic reticulum kinase) is encoded by the EIF2AK3 (also known as PEK, PERK, WRS) gene (Gene ID 9451) in human. The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) induces the activation of three main unfold protein response (UPR) pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) upregulation.

Immunogeno

Epitope: Internal (C-terminal half)

GST-tagged recombinant protein corresponding to the Internal (C-terminal half) of human PERK.

Applicazioni

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PERK in human liver tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-PERK Antibody, clone 4F6.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of PERK.

Qualità

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PERK in 10 µg of HEK293 cell lysate.

Descrizione del bersaglio

~125 kDa observed. Uncharacterized band(s) may appear in some lysates.

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable