MABN712
Anti-CCL2 Antibody, clone 2D8
clone 2D8, from mouse
Sinonimo/i:
C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
2D8, monoclonal
Reattività contro le specie
monkey, mouse, rat, human
tecniche
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG1
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... CCL2(6347)
Descrizione generale
CCL2, Chemokine (C-C motif) ligand 2, or alternatively known as Monocyte chemotactic protein 1 (MCP-1), Monocyte chemotactic and activating factor (MCAF), Monocyte chemoattractant protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, HC11, and C-C motif chemokine 2 is encoded by the human gene named CCL2/MCP1/SCYA2. CCL2 is a chemokine produced by endothelial cells that attracts memory T cells, monocytes, dendritic cells, and basophils but not neutrophils or eosinophils to sites of inflammation produced by either tissue inquiry or infection. CCL2 also bolsters monocytes anti-tumor activity, and because it is chemotactic for monocytes, CCL2 has been implicated in inflammatory diseases where monocytic invasions have occurred such as in psoriasis, rheumatoid arthritis and atherosclerosis. CCL2 is a bifunctional molecule as well; the whole molecule is a basophil activator but removal of the N-terminal portion converts the molecule into an exclusive eosinophil chemoattractant. CCL2 can also be found expressed at the sites of tooth eruption and bone degradation and is expressed by both osteoclasts and osteoblasts. CCL2 is also expressed in neurons, astrocytes and microglia and its role in neuroinflammation is established. CCL2 expression in glial and neurons is increased in epilepsy, brain ischemia, Alzheimer’s disease, brain injury and experimental autoimmune encephalomyelitis. Finally, CCL2 plays a role in vascular complications associated with type II diabetes and in the production of amylin which contributes to insulin resistance in obesity, which is also associated with type II diabetes. EMD-Millipore’s Anti-CCL2 mouse monoclonal antibody has been tested in western blot against recombinant protein and cell lysates from A549, HeLa, Raw264.7, L1210, C6, and COS7 cell lines.
Immunogeno
Purified recombinant fragment of human CCL2 expressed in E. Coli.
Applicazioni
Detect Monocyte Chemotactic Protein using this mouse monoclonal antibody, Anti-CCL2 Antibody, clone 2D8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected CCL2 in HeLa cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CCL2 in human liver cancer tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CCL2 in A549 cells.
Optimal working dilutions must be determined by end user.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CCL2 in human liver cancer tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CCL2 in A549 cells.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Qualità
Evaluated by Western Blotting in A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CCL2 in A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CCL2 in A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
Descrizione del bersaglio
~14 kDa observed. Uncharacterized bands may appear in some lysate(s).
Stato fisico
Format: Purified
Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Risultati analitici
Control
A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Non trovi il prodotto giusto?
Prova il nostro Motore di ricerca dei prodotti.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Lisha Luo et al.
Thoracic cancer, 13(6), 811-823 (2022-02-10)
Monocytes are involved in tumor growth and metastasis, but the distribution of monocyte phenotypes and their role in the development of malignant pleural effusion (MPE) remains unknown. A total of 94 MPE patients (76 diagnosed with adenocarcinoma lung cancer and
Milene Volpato et al.
Oncotarget, 7(19), 28139-28150 (2016-04-09)
The mechanism of the anti-colorectal cancer (CRC) activity of the omega-3 fatty acid eicosapentaenoic acid (EPA) is not understood. We tested the hypothesis that EPA reduces expression of chemokine C-C motif ligand 2 (CCL2), a pro-inflammatory chemokine with known roles
Xue Shi et al.
BMC neuroscience, 23(1), 20-20 (2022-04-01)
C-C motif chemokine ligand 2 (CCL2) is reported to be involved in the pathogenesis of various neurological and/or psychiatric diseases. Tissue or cellular expression of CCL2, in normal or pathological condition, may play an essential role in recruiting monocytes or
Ying Chen et al.
International journal of molecular sciences, 22(20) (2021-10-24)
Microglial activation is implicated in retinal vasoregression of the neurodegenerative ciliopathy-associated disease rat model (i.e., the polycystic kidney disease (PKD) model). microRNA can regulate microglial activation and vascular function, but the effect of microRNA-124 (miR-124) on retinal vasoregression remains unclear.
D Sarr et al.
Placenta, 36(7), 738-749 (2015-05-10)
Plasmodium chabaudi AS-infection in pregnant A/J and C57BL/6J mice results in mid-gestational pregnancy loss. Although associated with increased systemic and placental pro-inflammatory responses and coagulopathy, the molecular mechanisms that underlie poor pregnancy outcomes in these mice are not yet fully
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.