MABF235

Anti-AIRE Antibody, clone Mab155

clone Mab155, 1 mg/mL, from mouse

• Sinonimo/i: Autoimmune regulator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, APECED protein
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: Mab155, monoclonal
• Reattività contro le specie: human
• Concentrazione: 1 mg/mL
• tecniche: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_000374
• N° accesso UniProt: O43918
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... AIRE(326)

Descrizione

Descrizione generale

AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).

Immunogeno

His-tagged recombinant protein corresponding to human AIRE.

Applicazioni

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

This Anti-AIRE Antibody, clone Mab155 is validated for use in Flow Cytometry and Western Blotting and Immunocytochemistry for the detection of AIRE.

Western Blotting Analysis: A representative lot detected AIRE in Cos1 transfected cell lysate (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).
Immunocytochemistry Analysis: A representative lot detected AIRE in freshly isolated endogenous peripheral blood lymphocytes (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).

Qualità

Evaluated by Flow Cytometry in human PBMC cells.

Flow Cytometry Analysis: 0.5 µg of this antibody detected AIRE in human PBMC cells.

Descrizione del bersaglio

58 kDa calculated

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable