06-570-AF555

Anti-phospho-H3 Antibody (Ser10), Alexa Fluor^™ 555 Conjugate

from rabbit, ALEXA FLUOR^™ 555

• Sinonimo/i: H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Coniugato: ALEXA FLUOR^™ 555
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): mouse (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable
• N° accesso NCBI: NP_003484.1
• N° accesso UniProt: Q16695
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer10)
• Informazioni sul gene: human ... HIST1H3F(8968)

Descrizione

Descrizione generale

Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

Immunogeno

Linear peptide corresponding to phospho-Histone H3 (Ser10).

Applicazioni

Anti-phospho-H3 (Ser10), Alexa Fluor^™ 555 Conjugate is a purified rabbit polyclonal Antibody that detects Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 555 and validated for use in IC. The unconjugated version of this antibody is published & proven in ICC, IP & WB.

Immunoprecipitation Analysis: 10 µg from a representative lot of the non-conjugated form of this antibody (Cat. # 06-570) immunoprecipitated phospho-Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.

Beadlyte Assay Analysis: A representative lot of the non-conjugated form of this antibody (Cat. # 06-570) recognized Histone H3 phosphorylated on Ser10 by Luminex assay.

Western Blot Analysis: 0.5-1 µg/mL from a representative lot of the non-conjugated form of this antibody (Cat. # 06-570) detected phospho-Histone H3 (Ser10) on 10 µg of Colcemid-treated HeLa acid extract, but not on untreated HeLa acid extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Qualità

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.

Descrizione del bersaglio

~17 kDa observed

Stato fisico

Protein A purified

Purified rabbit polyclonal in buffer containing PBS with 15 mg/ml BSA and 0.1% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable