MABF2053

Anti-CD117 (c-Kit) Antibody, clone 2C11

clone 2C11, from mouse

• Sinonimo/i: Mast/stem cell growth factor receptor Kit, EC: 2.7.10.1, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 2C11, monoclonal
• Reattività contro le specie: mouse, human
• Confezionamento: antibody small pack of 25 μg
• tecniche: flow cytometry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_000213.1
• N° accesso UniProt: P10721
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... KIT(3815)

Descrizione

Descrizione generale

Mast/stem cell growth factor receptor Kit (UniProt: P10721; also known as EC: 2.7.10.1, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) is encoded by the KIT (also known as SCFR) gene (Gene ID: 3815) in human. C-Kit is a single-pass type I membrane protein with tyrosine protein kinase activity and serves as a cell-surface receptor for the cytokine KITLG/SCF and is shown to play a significant role in the regulation of cell survival, cell proliferation, stem cell maintenance, gametogenesis and other functions. c-Kit exists as a monomer in the absence of bound KITLG/SCF, however, it homodimerizes in the presence of bound KITLG/SCF and forms a heterotetramer with two KITLG/SCF molecules. c-Kit can be autophosphorylated on tyrosine residues and KITLG/SCF binding enhances its autophosphorylation. Its kinase activity is down-regulated by phosphorylation on serine residues by PKC family members. Phosphorylation at Tyrosine 568 is essential for its interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. c-Kit can be ubiquitinated by SOCS6 and it is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to its internalization and degradation. c-Kit is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form with an extracellular domain (aa 26-524), a short transmembrane domain (aa 525-545), and a cytoplasmic domain (aa 546-976. Its two ATP-binding sites are localized to amino acids 596-603 and 671-677. Lack of c-Kit expression has been reported in multiple cancer types and c-Kit expressing cells display a regression of typical morphological features of malignancy.

Specificità

Clone 2C11 specifically detects CD117 (c-Kit) in human and murine cells. It targets an epitope with in the extracellular domain.

Immunogeno

GST-tagged recombinant fragment corresponding to 195 amino acids from the extracellular domain of Human C-kit.

Applicazioni

Flow Cytometry Analysis: A representative lot detected CD117 (c-Kit) in P815 cells (Courtesy of the Monoclonal Antibody Unit at the Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore).

Western Blotting Analysis: A representative lot detected CD117 (c-Kit) in DLD-1 cell lysate (Courtesy of the Monoclonal Antibody Unit at the Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore).

This mouse monoclonal Anti-CD117 (c-Kit), clone 2C11 Antibody, Cat. No. MABF2053 is tested for use in Western Blotting and Flow Cytometry, for the detection of C-kit.

Qualità

Evaluated by Western Blotting in DLD-1 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CD117 (c-Kit) in DLD-1 cell lysate.

Descrizione del bersaglio

~160 kDa observed; 109.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable