MABC681

Anti-ETV4 Antibody, clone 1A2G3

ascites fluid, clone 1A2G3, from mouse

• Sinonimo/i: ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, E1A-F, Polyomavirus enhancer activator 3 homolog, Protein PEA3
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: 1A2G3, monoclonal
• application: WB
• Reattività contro le specie: human
• tecniche: western blot: suitable

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: 1A2G3, monoclonal
• Reattività contro le specie: human
• tecniche: western blot: suitable
• Isotipo: IgG1
• N° accesso UniProt: P43268
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ETV4(2118)

Descrizione

Descrizione generale

ETS translocation variant 4 protein, alternatively known as Adenovirus E1A enhancer-binding protein E1A-F, or Polyomavirus enhancer activator 3 homolog (PEA3), is encoded by the human gene named ETVA/E1AF/PEA3. ETV4 is a protein that is a transcriptional activator that was first recognized as such because it allowed for the expression of the adenovirus E1A gene upon infection. Today ETV4 has been shown to be active in a wide variety of biological processes from stem cell differentiation, to mammary gland and duct morphogenesis to motor neuron and axon guidance. ETV4 activity is regulated via the ERK/MAP signaling and a complex interplay of post-translation modifications such as sumoylation and ubiquitination. In cancers, ETV4 activates HIF and its subsequent proliferation genes and overexpression in prostate cells leads to oncogenesis. EMD-Millipore’s Anti-ETV4 mouse monoclonal antibody has been tested in western blot against K562 cell lysates as well as recombinantly expressed ETV4 protein.

Immunogeno

Purified recombinant fragment of human ETV4 expressed in E. Coli.

Applicazioni

Detect ETS translocation variant 4 protein using this mouse monoclonal antibody, Anti-ETV4 Antibody & clone 1A2G3 validated for use in western blotting.

Qualità

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ETV4 in K562 cell lysate.

Optimal working dilutions must be determined by end user.

Descrizione del bersaglio

~54 kDa observed. Uncharacterized bands may appear in some lysate(s).

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable