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MABC006F

Sigma-Aldrich

Mouse IgG2b Negative Control, clone GC198, FITC conjugate

Mouse IgG2b Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.42
Coniugato:
FITC conjugate
application:
FACS
IF
Clone:
GC198, monoclonal
tecniche:
flow cytometry: suitable
immunofluorescence: suitable
citations:
9

Origine biologica

mouse

Livello qualitativo

Coniugato

FITC conjugate

Forma dell’anticorpo

purified immunoglobulin

Clone

GC198, monoclonal

Produttore/marchio commerciale

Chemicon®

tecniche

flow cytometry: suitable
immunofluorescence: suitable

Isotipo

IgG2b

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Specificità

This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

Immunogeno

Protein I preparation from Neisseria gonorrhoeae.

Applicazioni

FITC conjugated mouse IgG2b is a monoclonal antibody intended for use as a negative control in flow cytometry. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
Mouse IgG2b Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies

Stato fisico

Purified immunoglobulin. Fluorescein conjugated, ready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Stoccaggio e stabilità

Store at 2 to 8oC, for up to 6 months. DO NOT FREEZE. Protect from light.

WARNING

The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Narges Labibzadeh et al.
Cell journal, 18(3), 302-309 (2016-09-08)
Nonunion is defined as a minimum of 9 months since injury without any visible progressive signs of healing for 3 months. Recent literature has shown that the application of mesenchymal stromal cells is safe, in vitro and in vivo, for
Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.
Beers, J; Gulbranson, DR; George, N; Siniscalchi, LI; Jones, J; Thomson, JA; Chen, G
Nature Protocols null
Vivek Shukla et al.
Cancer research, 77(22), 6267-6281 (2017-09-25)
In this study, we generated induced pluripotent stem cells (iPSC) from normal human small airway epithelial cells (SAEC) to investigate epigenetic mechanisms of stemness and pluripotency in lung cancers. We documented key hallmarks of reprogramming in lung iPSCs (Lu-iPSC) that

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