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MAB5448

Sigma-Aldrich

Anti-Neurofilament H (200 kDa) Antibody

CHEMICON®, rat monoclonal, TA51

Sinonimo/i:

NF-H

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-Neurofilament NF-H Antibody, clone TA51, culture supernatant, clone TA51, Chemicon®

Origine biologica

rat

Livello qualitativo

Forma dell’anticorpo

culture supernatant

Tipo di anticorpo

primary antibodies

Clone

TA51, monoclonal

Reattività contro le specie

human, rat, hamster, monkey

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... NEFH(4744)
rat ... Nefh(24587)
rhesus monkey ... Nefh(717705)

Specificità

Neurofilament H (NF-H), carboxy terminal (phosphate dependent (+). Clone is specific for phosphorylated NF-H {Carden et al (1987) 7(11):3489}.

Immunogeno

Epitope: carboxy terminal phosphorylation dependent, within the multiphosphorylation repeat in NFH

Applicazioni

Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
Western blot: Identifies ~ 200kDa band in reduced samples. Use Tris based buffers for best results as epitope is phosphate dependent; Different regions of the CNS were dissected out immediately and homogenized in BUST buffer (8 M urea, 0.5% SDS, 2% 13-mercaptoethanol, and 50 mM Tris, pH 7.4) at a ratio of i ml/100 mg wet tissue. The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g

for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640}

Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}.

Optimal working dilutions must be determined by end user.

Stato fisico

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

Stoccaggio e stabilità

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Annals of neurosciences, 18(2), 48-55 (2011-04-01)
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Proceedings of the National Academy of Sciences of the USA, 93, 3155-3160 (1996)
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