MAB4385

Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA-1-85

clone TRA-1-85, Chemicon^®, from mouse

• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: TRA-1-85, monoclonal
• Reattività contro le specie: human
• Produttore/marchio commerciale: Chemicon^®
• tecniche: flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• input: sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• Isotipo: IgG1
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells.

Specificità

This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed.

SPECIES REACTIVITIES:

Refer to comments regarding specificity

Immunogeno

2102Ep human Embryonal Carcinoma Cells

Epitope: blood group antigen Ok(a)

Applicazioni

Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB.

FACS Analysis: a starting range of 5-20 μg/ml is suggested.

Immunoprecipitation

Immunoblotting

Optimal working dilutions must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Descrizione del bersaglio

35-69 kDa

Stato fisico

Format: Purified

Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide

Stoccaggio e stabilità

Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months

Risultati analitici

Control
Hemopoietic cells and a wide range of normal human tissues

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable