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Merck
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Key Documents

MAB4301C3

Sigma-Aldrich

Anti-SSEA1 Antibody, clone MC-480, Cy3 conjugate

clone MC-480, from mouse, CY3 conjugate

Sinonimo/i:

SSEA-1, SSEA1, CD15, Lewis X, 3-FAL

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

CY3 conjugate

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

MC-480, monoclonal

Reattività contro le specie

human

tecniche

immunocytochemistry: suitable

Isotipo

IgM

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... FUT4(2526)

Descrizione generale

Stage-specific embryonic antigen-1 (SSEA-1) is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Specificità

This antibody recognizes mouse Stage-Specific Embryonic Antigen-1 (SSEA-1).

Immunogeno

F9 teratocarcinoma stem cells (X-irradiated).

Applicazioni

Detect SSEA1 using this Anti-SSEA1 Antibody, clone MC-480, Cy3 conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected no SSEA-1 in H9 human embryonic stem cells.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Glycobiology

Qualità

Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected SSEA-1 in mouse embryonic stem cells (SCR012).

Descrizione del bersaglio

The unconjugated parent antibody (Catalog No. MAB4301) has an observed MW at 220-240 kDa

Stato fisico

Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Stoccaggio e stabilità

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Risultati analitici

Control
Mouse embryonic stem cells (SCR012).

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Bo Feng et al.
Nature cell biology, 11(2), 197-203 (2009-01-13)
The dominant effect of transcription factors in imparting expanded potency is best exemplified by the reprogramming of fibroblasts to pluripotent cells using retrovirus-mediated transduction of defined transcription factors. In the murine system, Oct4, Sox2, c-Myc and Klf4 are sufficient to
Establishment and characterization of embryonic stem-like cells from porcine somatic cell nuclear transfer blastocysts.
Kim S, Kim JH, Lee E, Jeong YW, Hossein MS, Park SM, Park SW, Lee JY, Jeong YI, Kim HS et al.
Zygote null
Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes.
Sung LY, Chang CC, Amano T, Lin CJ, Amano M, Treaster SB, Xu J, Chang WF, Nagy ZP, Yang X, Tian XC
Cellular Reprogramming null

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