MAB2014

Anti-OB-Cadherin Antibody, extracellular domain, clone 16A

clone 16A, Chemicon^®, from mouse

• Sinonimo/i: Cadherin-11
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 16A, monoclonal
• Reattività contro le specie: rat, human
• Produttore/marchio commerciale: Chemicon^®
• tecniche: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG1
• N° accesso NCBI: NM_001797.2
• N° accesso UniProt: P55287
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CDH11(1009)

Descrizione

Descrizione generale

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.

Specificità

Reacts with the extracellular domain of human cadherin-11.

Immunogeno

Epitope: extracellular domain

Fusion protein corresponding to extracellular domain of human OB-cadherin coupled to GST.

Applicazioni

Detect OB-Cadherin using this Anti-OB-Cadherin Antibody, extracellular domain, clone 16A validated for use in WB, IC, IH.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western blot: 1:25 - 1:250
Immunohistochemistry: Frozen tissue sections. 1:25 - 1:50 dilution using avidin-biotinylated horseradish peroxidase complex (ABC) as detection protocol.
Immunocytochemistry: Methanol and paraformaldehyde-fixed cells
Optimal working dilutions must be determined by the end user.I/

Stato fisico

Affinity purified immuoglobulin. Liquid in PBS containing 0.09% sodium azide.

Format: Purified

Stoccaggio e stabilità

1 year at 4°C from date of shipment

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable