MAB1563
Anti-Presenilin-1 Antibody, NT, clone hPS1-NT
culture supernatant, clone hPS1-NT, Chemicon®
Sinonimo/i:
Anti-ACNINV3, Anti-FAD, Anti-PS-1, Anti-PS1, Anti-S182
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rat
Livello qualitativo
Forma dell’anticorpo
culture supernatant
Tipo di anticorpo
primary antibodies
Clone
hPS1-NT, monoclonal
Reattività contro le specie
human
Produttore/marchio commerciale
Chemicon®
tecniche
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2a
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... PSEN1(5663)
Specificità
Recognizes Presenilin-1, human. By Western blot the antibody recognizes a predominant 32 kDa polypeptide in a variety of samples, including PC12 cells transfected with human PS1 complementary DNA, brain biopsy specimens from demented patients, and postmortem samples of frontal neucortex from Familial Alzheimer′s Disease cases, late-onset Alzheimer′s disease cases, and cases of other degenerative disorders. Immunohistochemical studies of control brains revealed that PS1 is expressed primarily in neurons, with the protein localizing in the soma and dendritic processes. In contrast in FAD and sporadic Alzheimer′s disease cases, PS1 immunoreactivity was present in the neuritic component of senile plaques as well as in neurofibrillary tangles. Localization of PS1 immunoreactivity in familial and sporadic Alzheimer′s disease suggest that genetically heterogeneous forms of the disease share a common pathophysiology involving PS1 protein.
Immunogeno
A fusion protein antigen containing the N-terminus of human PS-1 (residues 21-80) fused to GST.
Epitope: N-terminus
Applicazioni
Immunohistochemistry: rat pups and 24 old month rats were perfused for 10 minutes with 100-200mL of 4% PFA 0.3% glutaraldehyde and 0.1% CaCl2 in 0.1M PBS pH 7.4. Vibratome sections were blocked with 3% normal serum and permeabilized with 0.1% triton X-100.{Neuroscience 2003 120:405-423}.
Immunoblotting: 1:250-1:500. Recommend blocking buffer is TBS containing 5% non fat milk and 0.01% Tween 20. It is recommended that you also dilute the antibody in this blocking buffer. Incubate with the MAB1563 for 1 to 2 hours at room temperature or overnight at 4°C.
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunoblotting: 1:250-1:500. Recommend blocking buffer is TBS containing 5% non fat milk and 0.01% Tween 20. It is recommended that you also dilute the antibody in this blocking buffer. Incubate with the MAB1563 for 1 to 2 hours at room temperature or overnight at 4°C.
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Presenilin-1 Antibody, N-terminus, clone hPS1-NT is validated for use in ELISA, IH, IP, WB for the detection of Presenilin-1.
Descrizione del bersaglio
32 kDa
Stato fisico
UnPurified mouse tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Unpurified
Stoccaggio e stabilità
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Risultati analitici
Control
Brain tissue
Brain tissue
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
C Ribaut-Barassin et al.
Synapse (New York, N.Y.), 35(2), 96-110 (1999-12-28)
Healthy brain neurons co-express Alzheimer's disease (AD) related proteins presenilins (PS) and beta-amyloid precursor protein (beta-APP). Deposition of beta-amyloid and PS in the senile plaques of AD brain and their ability to interact in vitro suggest that AD pathology could
Increased tau phosphorylation but absence of formation of neurofibrillary tangles in mice double transgenic for human tau and Alzheimer mutant (M146L) presenilin-1.
A Boutajangout, K Leroy, N Touchet, M Authelet, V Blanchard, G Tremp, L Pradier, J P Brion
Neuroscience Letters null
Reduced antioxidant enzyme activity in brains of mice transgenic for human presenilin-1 with single or multiple mutations.
S Leutner, C Czech, K Schindowski, N Touchet, A Eckert, W E Muller
Neuroscience Letters null
Failure of the interaction between presenilin 1 and the substrate of gamma-secretase to produce Abeta in insect cells.
Didier Pitsi, Pascal Kienlen-Campard, Jean-Noel Octave
Journal of Neurochemistry null
Impact of aging and age-related maculopathy on activation of the a-wave of the rod-mediated electroretinogram.
Gregory R Jackson, Gerald McGwin, Janice M Phillips, Ronald Klein, Cynthia Owsley
Investigative Ophthalmology & Visual Science null
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