GF141

Stem Cell Factor Protein, Recombinant mouse

The Stem Cell Factor (SCF) Protein, Recombinant mouse, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.

• Sinonimo/i: SCF, Mast Cell Growth Factor, MGF, c-kit Ligand
• Codice UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Ricombinante: expressed in E. coli
• Saggio: >98% (SDS-PAGE)
• Attività specifica: ≥1x10e5 U/mg
• Produttore/marchio commerciale: Chemicon^®
• Impurezze: <0.1 ng/μg Endotoxin (of SCF)
• input: sample type hematopoietic stem cell(s)
• N° accesso UniProt: P20826; P21583
• Condizioni di spedizione: dry ice
• Informazioni sul gene: mouse ... KITLG(17311)

Descrizione

Descrizione generale

Product Source: E. coli

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Murine SCF is an 18.3 kDa protein containing 164 amino acid residues.

Applicazioni

Concentration Range for most in vitro applications is 1.0 - 100 ng/mL. Optimal working dilutions must be determined by end user.

The Stem Cell Factor (SCF) Protein, Recombinant mouse, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.

Linkage

Replaces: GF049

Stato fisico

Lyophilized protein from 10 mM Sodium Phosphate, pH 6.5, containing no additives.

Stoccaggio e stabilità

The lyophilized powder, though stable at room temperature, is best stored at -20°C for up to 12 months from date of receipt. After a quick spin, reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for one week or at -20°C for future use. Reconstituted mouse SCF should be stored in working aliquots at -20°C for up to six months. Avoid repeated freeze/thaw cycles.

Risultati analitici

Specific Activity: Murine SCF is biologically active when compared to standards.The ED(50), as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cell line is ≤ 10 ng/mL

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2