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Key Documents

FCMAB230P

Sigma-Aldrich

Milli-Mark® Mouse IgG1-k, clone MOPC-21, PE conjugate

This Milli-Mark Anti-mouse IgG1-PE Antibody, clone MOPC-21 is validated for use in FC for the detection of mouse IgG1.

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

PE

Forma dell’anticorpo

purified immunoglobulin

Clone

MOPC-21, monoclonal

Reattività contro le specie

human

Produttore/marchio commerciale

Milli-Mark®

tecniche

flow cytometry: suitable

Isotipo

IgG1κ

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

The MOPC-21 tumor line that produces mouse IgG1,k, is a mineral oil induced plasmacytoma. The product is originated and carried intraperitoneally in BALB/c mice. Identity and purity of the immunoglobulin is established by immunoelectrophoresis (IEP) and Ouchterlony Double Diffusion (ODD), prior to conjugation. Electrophoresis of the purified immunoglobulin, followed by diffusion versus anti-mouse whole serum, antimouse IgG1 and anti-mouse kappa chain, results in single arcs of precipitation. By ODD the purified immunoglobulin preparation is non-reactive with antisera to mouse lambda light chain, mouse IgA, IgM, IgG2a, IgG2b, and IgG3. The specificity of staining by monoclonal antibodies to human CD antigens should be verified by establishing the amount of non-specific binding to the target cell population. It is recommended that a non-reactive immunoglobulin of the same isotype, concentration and F/P molar ratio be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.

Specificità

This mouse IgG1 isotype control antibody is not reactive with any known antigen.

Applicazioni

Research Category
Inflammation & Immunology
Research Sub Category
Isotype Control Antibodies

Qualità

Evaluated for use as flow cytometry isotype control using Jurkat Cells.

Stato fisico

Immunoelectrophoresis
Purified mouse monoclonal IgG1κ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Stoccaggio e stabilità

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Note legali

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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