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Key Documents

AP1063

Sigma-Aldrich

PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb

liquid, Calbiochem®

Sinonimo/i:

Anti-Pyruvate Dehydogenase pSer²³² Rabbit pAb

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

mouse, human, rat

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

phosphorylation (pSer232)

Descrizione generale

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells.
This PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²).

Immunogeno

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α

Applicazioni



Immunoblotting (0.25 g/ml)
Immunofluorescence (Please see comments)
Immunoprecipitation (1 g/ml)

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In PBS.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Risultati analitici

Positive Control
HEK293 cells

Altre note

Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
This antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Science translational medicine, 5(175), 175ra31-175ra31 (2013-03-08)
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