ABT52

Anti-phospho-Peak Antibody (Tyr665)

from rabbit, purified by affinity chromatography

• Sinonimo/i: Pseudopodium-enriched atypical kinase 1, Sugen kinase 269, Tyrosine-protein kinase SgK269
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• application: DB; WB
• Reattività contro le specie: human
• tecniche: dot blot: suitable; western blot: suitable
• citations: 2

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): porcine (based on 100% sequence homology), rat (based on 100% sequence homology), orangutan (Sumatran; based on 100% sequence homology), mouse (based on 100% sequence homology)
• tecniche: dot blot: suitable; western blot: suitable
• N° accesso NCBI: NP_079052
• N° accesso UniProt: Q9H792
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pTyr665)
• Informazioni sul gene: human ... PEAK1(79834)

Descrizione

Descrizione generale

Pseudopodium-enriched atypical kinase 1 (Peak1; KIAA2002, or sgk269) is a ubiquitous non-receptor tyrosine kinase that belongs to the NFK3 family. It interacts with actin filaments via its N-terminal actin-targeting region. It also modulates the activity of multiple cytoskeletal regulatory proteins such as paxillin, p130Cas, Erk, and Crk; and mediates cell proliferation and migration via the Ras/Raf/Erk and Src-p130Cas-Crk-Paxillin pathways. Peak1 may play an important role in the progression of tumors.

Specificità

This antibody recognizes Peak phosphorylated at Tyr665.

Immunogeno

Epitope: Phosphorylated Tyr665

KLH-conjugated linear peptide corresponding to human Peak phosphorylated at Tyr665.

Applicazioni

This phospho-Peak antibody is validated for use in WB & Dot Blot for the detection of the phospho-Peak protein.

Western Blot Analysis: A representative lot was used by an independent laboratory in 293T cells transfected with wildtype GFP-Peak. (Dr. Richard Klemke, University of California-San Diego.)

Qualità

Evaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins.

Dot Blot (Specificity) Analysis: A representative lot blocked phospho-Peak (Tyr665) in modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins.

Descrizione del bersaglio

220 kDa calculated

Risultati analitici

Control
Modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable