Passa al contenuto
Merck
Tutte le immagini(2)

Documenti fondamentali

ABT115

Sigma-Aldrich

Anti-FucT-III Antibody

from rabbit, purified by affinity chromatography

Sinonimo/i:

Galactoside 3(4)-L-Fucosyltransferase, Blood Group Lewis Alpha-4-Fucosyltransferase, Lewis Ft, Fucosyltransferase 3, Fucosyltransferase Iii, Fuct-Iii, Alpha-(1,3)-Fucosyltransferase 3

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
application:
WB
Reattività contro le specie:
human
tecniche:
western blot: suitable
citations:
1

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

human

Reattività contro le specie (prevista in base all’omologia)

chimpanzee (based on 100% sequence homology)

tecniche

western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... FUT3(2525)

Descrizione generale

Alpha-(1,3)-fucosyltransferase 3 (FucT-III; Galactoside 3(4)-L-fucosyltransferase) is highly expressed in the small intestine, colon, stomach, and kidney. It is a member of the glycosyltransferase family of proteins which are involved in a glycosylation pathway that attaches fucose to Lewis epitopes of target molecules. This type of post-translational modification allows modified (fucosylated) molecules to recognize specific target substrates. Fucosyltransferases may therefore play an important role in communication at the cell surface. FucT-III may play a role in blood group determination as it is involved in the expression of antigens such as Lewis B, sialy Lewis X, Vim-2, and Lewis A.

Specificità

No homology to Rat and Mouse was found.
This antibody recognizes FucT-III at the lumenal region.

Immunogeno

Epitope: Lumenal region
KLH-conjugated linear peptide corresponding to the lumenal region of human FucT-III.

Applicazioni

Anti-FucT-III Antibody detects level of FucT-III & has been published & validated for use in Western Blotting.
Research Category
Cell Structure
Research Sub Category
Glycobiology
Western Blot Analysis: 1 µg/mL from a representative lot detected FucT-III on 10 µg of transfected lysate and did not detect the non transfected lysate.

Qualità

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected FucT-III on 10 µg of human liver tissue lysate.

Descrizione del bersaglio

~50 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~50 kDa due to high glycosylation. (Christensen, L, et al. (2000). Glycobiology. 10(9):931-939.)

Stato fisico

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Human Liver Tissue Lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Non trovi il prodotto giusto?  

Prova il nostro Motore di ricerca dei prodotti.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

L L Christensen et al.
Glycobiology, 10(9), 931-939 (2000-09-16)
The alpha1,3/4-fucosyltransferases are involved in the synthesis of fucosylated cell surface glycoconjugates. Human alpha1,3/4-fucosyltransferase III, -V, and -VI (hFucTIII, -V, and -VI) contain two conserved C-terminal N-glycosylation sites (hFucTIII: Asn154 and Asn185; hFucTV: Asn167 and Asn198; and hFucTVI: Asn153 and

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.