ABS310

Anti-hCE-1 Antibody

serum, from rabbit

• Sinonimo/i: Carboxylesterase 1, Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, ACAT, Brain carboxylesterase hBr1, CE-1, hCE-1, Cocaine carboxylesterase, Egasyn, HMSE, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine estera
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human
• tecniche: western blot: suitable
• N° accesso NCBI: NP_001020365.1
• N° accesso UniProt: P23141
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CES1(1066)

Descrizione

Descrizione generale

Carboxylesterase 1 (hCE-1) is also known as Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, (ACAT), Cocaine carboxylesterase, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, (REH), Serine esterase 1, and Triacylglycerol hydrolase (TGH). hCE-1 is involved in: the detoxification of xenobiotics and the activation of ester and amide prodrugs. hCE-1 functions to hydrolize the methyl ester group of cocaine to form benzoylecgonine, catalyze the transesterification of cocaine to form cocaethylene and the ethyl esterification of oleic acid to ethyloleate, and is activated by CHAPS. hCE-1 is expressed mostly in liver with lower levels found in the heart and lung.

Specificità

This antibody does not cross react with human CE-2 (Prof. P. Potter, St. Jude Children′s Research Hospital, Memphis).

Immunogeno

Linear peptide corresponding to human hCE-1.

Applicazioni

Detect Carboxylesterase 1 using this rabbit polyclonal antibody, Anti-hCE-1 Antibody validated for use in western blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected hCE-1 in 10 µg of human liver tissue lysate.
Western Blotting Analysis: A representative lot from an independent laboratory detected hCE-1 in hCE-1 transfected U373MG cell lysates, and demonstrated a loss of signal in non-transfected and hCE-2 transfected U373MG cell lysates (Prof. P. Potter, St. Jude Children′s Research Hospital, Memphis.).

Qualità

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected hCE-1 in 10 µg of HepG2 cell lysate.

Descrizione del bersaglio

~58 kDa observed. A homodimeric trimer of three hCE-1 molecules may be observed at ~190 kDa in some cell lysates (Prof. P. Potter, St. Jude Children′s Research Hospital, Memphis.).

Stato fisico

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
HepG2 cell lysate

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1