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Merck
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Key Documents

MABF288

Sigma-Aldrich

Anti-Roquin-1/2, clone 3F12 Antibody

clone 3F12, from rat

Sinonimo/i:

Roquin-1, Roquin, RING finger and C3H zinc finger protein 1, RING finger and CCCH-type zinc finger domain-containing protein 1, RING finger protein 198

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rat

Livello qualitativo

100

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

3F12, monoclonal

Reattività contro le specie

human, mouse

tecniche

immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2aκ

N° accesso NCBI

NP_742068

N° accesso UniProt

Q5TC82

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... RC3H1(149041)

Descrizione generale

Roquin-1 shares 46% identity with Roquin-2 and both serve the same function and belong to the Zinc finger family of RNA binding proteins. Roquin-1/2 are cytoplasmic RNA suppression proteins that specifically bind selective RNAs and promote the deadenylation of mature mRNAs and their degradation. Roquin-1/2s targets include HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more critical RNAs. In particular, because of their impact on ICSO and gamma interferon and T helper cell activation, Roquin-1/2 are being examined as mediators of the systemic inflammation response and controllers of adaptive and innate immune responses in autoimmune disease. Roquin 1/2 are widely expressed and localized in the cytoplasm and/or P bodies and cytosolic stress granules when under cellular stress.

Specificità

This antibody specifically detects both Roquin-1 & Roquin-2 (Vogel, K.U., et al. (2013). Immunity. 38:655-668).

Immunogeno

Recombinant protein corresponding to human Roquin-1/2.

Applicazioni

This Anti-Roquin-1/2, clone 3F12 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Roquin-1/2.
Western Blotting Analysis: A representative lot detected Roquin-1/2 in both Roquin-1 & Roquin-2 in normal MEFs, only Roquin-2 and Roquin-1 deficient MEFs, and neither protein in Roquin-1/2 deficient MEFs. This antibody also detected Roquin-1 and Roquin-2 in Mouse Spleen, Lymph Node, Thymus, Brain & Lung tissue, CD4+ T cells and Thymocyte lysates (Vogel, K.U., et al. (2013). Immunity. 38:655-668).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Roquin-1/2 in MEF cell lysate (Vogel, K.U., et al. (2013). Immunity. 38:655-668).

Descrizione del bersaglio

125 kDa calculated

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Cleavage of roquin and regnase-1 by the paracaspase MALT1 releases their cooperatively repressed targets to promote TH17 differentiation.
Jeltsch, KM; Hu, D; Brenner, S; Zoller, J; Heinz, GA; Nagel, D; Vogel, KU; Rehage et al.
Nature Immunology null
Johannes Braun et al.
Nucleic acids research, 46(22), 12109-12125 (2018-10-09)
Post-transcriptional gene regulation controls the amount of protein produced from a specific mRNA by altering both its decay and translation rates. Such regulation is primarily achieved by the interaction of trans-acting factors with cis-regulatory elements in the untranslated regions (UTRs)
Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation.
Vogel, KU; Edelmann, SL; Jeltsch, KM; Bertossi, A; Heger, K; Heinz, GA; Zoller, J; Warth et al.
Immunity null

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