ABS1509

Anti-phospho-Neph1 (Tyr637/638) Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1, Nephrin-like protein 1, phospho-Neph1 (Tyr637/638)
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• tecniche: western blot: suitable
• N° accesso NCBI: NP_001164456
• N° accesso UniProt: Q80W68
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pTyr637/pTyr638 )
• Informazioni sul gene: human ... KIRREL1(55243)

Descrizione

Descrizione generale

Kin of IRRE-like protein 1 (UniProt Q80W68; also known as Nephrin-like protein 1, Kin of IRRE like 1, Kin of irregular chiasm-like protein 1) is encoded by the Kirrel (also known as 6720469N11Rik, Kirrel1, Neph1) gene (Gene ID 170643) in murine species. Podocytes are specialized epithelial cells that are critical components of the glomerular filtration barrier. Glomerular injury, such as that caused by renal ischemia, is often characterized by the effacement of podocytes, loss of slit diaphragms, and proteinuria. The podocyte proteins Neph1 and nephrin are critical for maintaining the structural integrity of slit diaphragm and therefore the filtration function of the glomerulus by organizing a signaling complex at the podocyte cell membrane. The cytoplasmic domain of Neph1 plays a key role in actin cytoskeleton remodeling events that are directly associated with Fyn-mediated Neph1 pTyr637/638 phosphorylation that leads to the recruitment of adapter proteins and signaling molecules, such as Grb2, Csk tyrosine kinase, and ZO-1. Phosphorylation-induced association between Neph1 and ZO-1 plays a key role in defining the tight junction formation in podocytes.

Specificità

Mouse Neph1 Tyr637/638 site is equivalent to human Neph1 Tyr605/606. Equivalent target site is also present in human spliced isoforms 2&3 (Tyr621/622 & Tyr502/503, respectively), but not in murine spliced isoform 2.

Immunogeno

Epitope: Cytoplasmic domain

Linear peptide corresponding to the cytoplasmic domain of mouse phospho-Neph1 (Tyr637/638).

Applicazioni

Detect Neph1 using this rabbit Polyclonal Anti-phospho-Neph1 Antibody (Tyr637/638), Cat. No. ABS1509, validated for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A representative lot detected Neph1 phosphorylation in kinase assay using Fyn and COS-7 lysate containing exogenously expressed Neph1 (Data courtesy of Deepak Nihalini, University of Pennsylvania)
Western Blotting Analysis: A representative lot detected Neph1 phosphorylation in lysates from puromycin aminonucleoside-/PAN-treated human pocodytes (Arif, E., et al. (2014). J Biol Chem. 289(14):9502-9518).

Qualità

Evaluated by Western Blotting in pervanadate treated human podocyte cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neph1 phosphorylation at the target tyrosine residues in 7.5 µL of pervanadate treated human podocyte cell lysate.

Descrizione del bersaglio

~120 kDa observed. Target band size appears larger than the calculated molecular weight (87.2 kDa/murine isoform 1, 72.4-85.0 kDa/human isoform 1-3) due to posttranslational modifications (glycosylation & phosphorylation). Uncharacterized band(s) may appear in some lysates.

Stato fisico

Affinity purified

Purified rabbit polyclonal antibody in buffer containing PBS/glycine/5% BSA, .02% Sodium Azide and 30% glycerol.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3