ABS1028

Anti-GPR56, C-terminal Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: GPR56 seven-transmembrane subunit, GPR56 7TM, GPR56 subunit beta, GPR56 CT, GPR56©, TM7XN1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: rat, human
• Reattività contro le specie (prevista in base all’omologia): mouse (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_001185823
• N° accesso UniProt: Q8K209
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GPR56(9289)

Descrizione

Descrizione generale

G-protein coupled receptor 56 (GPR56) is a ubiquitous adhesion receptor, which belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is abundantly expressed in the in thyroid gland, brain, heart, and various tumor cells. Previous studies have suggested that GPR56 may inhibit the progression and metastasis of melanomas by interaction with extracellular-matrix proteins, such as Tissue Transglutaminase. GPR56 also plays a role in regulating VEGF production and angiogenesis via a PKCα-mediated pathway. GPR56 also plays a critical role in the development of the frontal cortex; previous studies have reported that mutations in GPR56 result in disorganized cortical lamination, which is most pronounced in the frontal cortex. This condition is known as bilateral frontoparietal polymicrogyria or BFPP.

Immunogeno

linear peptide corresponding to the C-terminus of Mouse GPR56.

Applicazioni

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipated GPR56 from rat kidney cell lysate and visualized through western blot (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Western Blot Analysis: A representative lot of this antibody detected GPR56 from rat kidney cell lysate (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Immunocytochemistry Analysis: A representative lot of this antibody detected GPR56 from transfected HEK293 cells (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)

This Anti-GPR56, C-terminal Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of GPR56, C-terminal.

Qualità

Evaluated by Western Blotting in Hela cell lysate.

Western Blotting Analysis: 1 µg/ml of this antibody detected GPR56 in 10 µg of Hela cell lysate.

Descrizione del bersaglio

~ 120 kDa observed
This receptor is heavily glycosylated in cells, and thus the apparent molecular mass of the full-length receptor in most cell types is around 90-120 kDa Moreover, this receptor is prone to extensive post-translational processing and proteolysis, and thus depending on the cell type the most prominent immunoreactive bands on Western blot are usually around 45 kDa, 25 kDa and/or 15 kDa in size

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable