ABF210

Anti-MDA5 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: MDA5 Antibody, Melanoma differentiation-associated protein 5, Interferon-induced with helicase C domain protein 1, IFIH1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso UniProt: Q9BYX4
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... IFIH1(64135)

Descrizione

Descrizione generale

Melanoma differentiation-associated protein 5 (MDA5), also known as Interferon-induced helicase C domain-containing protein 1 (IFIH1) is a member of the helicase family (RLR subfamily). MDA5 plays an important role in recognizing various viral components and triggers antiviral responses. When stimulated by dsRNA, MDA5 recruits adaptor protein VISA causing the activation of IRF-3 and NF-κB. MDA5 is widely expressed at low levels with barely detectable levels found in , testis and lung. Mutations in MDA5 have been associated with diabetes mellitus insulin-dependent type 19.

Specificità

This antibody recognizes the C-terminus of MDA5.

Immunogeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human MDA5.

Applicazioni

Detect Melanoma differentiation-associated protein 5 using this rabbit polyclonal antibody, Anti-MDA5 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected MDA5 in human lymph node tissue lysate.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected MDA5 in human lymph node cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Qualità

Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MDA5 in 15 µg of Daudi cell lysate.

Descrizione del bersaglio

~117 kDa observed. Uncharacterized bands may appear in some lysates.

Linkage

Replaces: MABF198

Stato fisico

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Daudi cell Lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable