ABE1865
Anti-UTX (KDM6A)
from rabbit, purified by affinity chromatography
Sinonimo/i:
Lysine-specific demethylase 6A, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
human, mouse
tecniche
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
ambient
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... KDM6A(7403)
Descrizione generale
Lysine-specific demethylase 6A (UniProt O15550; also known as Histone demethylase UTX, KDM6A, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein) is encoded by the KDM6A (also known as KABUK2, UTX) gene (Gene ID 7403) in human KDM6A associates with H3K4 methyltransferase MLL3/4 complex and specifically demethylates Lys-27 of histone H3 and plays a central role in histone code. It is shown to demethylate trimethylated and dimethylated, but not monomethylated H3 Lys-27. KDM6A mediates muscle regeneration through it demethylase activity, while it regulates mdsoderm differentiation of embryonic stem cells and mammary gland development independent of its demethylase activity. KDM6A also plays a potential role in regulating brown adipocyte thermogenic program. It is up-regulated during brown adipocyte differentiation and by cold exposure in both brown and white adipose tissue in mice, indicating its potential role in thermogenesis. Inactivation of KDM6A down-regulates brown fat specific gene expression. Mutations in KDM6A gene are a causative factor in Kabuki syndrome that is characterized by congenital mental retardation, postnatal dwarfism, large prominent earlobes, a cleft or high-arched palate, scoliosis, and other abnormalities.
Ref.:
Lederer, D et al. (2012). Am. J. Hum. Genet. 90, 119-124.
Zha, L et al. (2015). J. Biol. Chem. 290, 25151-25163.
Ref.:
Lederer, D et al. (2012). Am. J. Hum. Genet. 90, 119-124.
Zha, L et al. (2015). J. Biol. Chem. 290, 25151-25163.
Specificità
This polyclonal antibody detected the expected UTX target band in wild-type, but not Utx-knockout murine ESC nuclear extract (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Immunogeno
His-tagged recombinant fragment within the central region of human UTX (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).
Applicazioni
Anti-UTX (KDM6A), Cat. No. ABE1865, is a highly specific rabbit polyclonal antibody that targets Lysine-specific demethylase 6A and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western blotting.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected UTX (KDM6A) recruiment to the Myog, CKm, and Tnnc2 promoters following myogenic differentiation of primary murine myoblasts from wild-type or Utx(KI/KI) female mice harboring homozygous demethylase dead Utx mutant gene knock-in (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected a time-dependent UTX (KDM6A) enrichement at the Myogenin and CKm regulatory regions following myogenic differentiation of murine C2C12 myoblasts. shRNA-mediated Six4 knockdown, but not p38 MAPK inhibition, downregulated UTX (KDM6A) recruitment to the Myogenin and CKm loci (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).
Immunocytochemistry Analysis: A representative lot detected UTX (KDM6A) immunoreactivity by indirect immunofluorescence staining of 1% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized myofibers isolated from mouse extensor digitorum longus (EDL) muscles (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Immunofluorescence Analysis: A representative lot detected UTX (KDM6A) immunoreactivity by indirect immunofluorescence staining of 4% paraformaldehyde-fixed, 1% Triton X-100-permeabilized, transverse OCT cryosections sections of mouse tibialis anterior skeletal muscle (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated PTIP and RbBP5 with UTX using embryonic stem cell (ESC) nuclear extracts from wild-type or Utx(KI/KI) mice harboring homozygous demethylase dead Utx mutant gene knock-in. No UTX/PTIP/RbBP5 complex was precipitated from Utx-knockout ESCs (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Six4 with UTX (KDM6A) using nuclear extract from murine C2C12 myoblasts 24 hrs post myogenic differentiation (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).
Western Blotting Analysis: A representative lot detected UTX4 in myoblast whole cell lysates and embryonic stem cell (ESC) nuclear extracts from wild-type or Utx(KI/KI) mice harboring homozygous demethylase dead Utx mutant gene knock-in, as well as in UTX and MLL4 immunoprecipitates. No UTX target band was detected in Utx-knockout ESCs (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Western Blotting Analysis: A representative lot detected UTX (KDM6A) in nuclear extracts from human K562 and murine C2C12 myoblasts (24 hrs post myogenic differentiation), as well as in Six4 immunoprecipitates. shRNA treatment prior to nuclear extract preparation diminished target band detection (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected a time-dependent UTX (KDM6A) enrichement at the Myogenin and CKm regulatory regions following myogenic differentiation of murine C2C12 myoblasts. shRNA-mediated Six4 knockdown, but not p38 MAPK inhibition, downregulated UTX (KDM6A) recruitment to the Myogenin and CKm loci (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).
Immunocytochemistry Analysis: A representative lot detected UTX (KDM6A) immunoreactivity by indirect immunofluorescence staining of 1% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized myofibers isolated from mouse extensor digitorum longus (EDL) muscles (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Immunofluorescence Analysis: A representative lot detected UTX (KDM6A) immunoreactivity by indirect immunofluorescence staining of 4% paraformaldehyde-fixed, 1% Triton X-100-permeabilized, transverse OCT cryosections sections of mouse tibialis anterior skeletal muscle (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated PTIP and RbBP5 with UTX using embryonic stem cell (ESC) nuclear extracts from wild-type or Utx(KI/KI) mice harboring homozygous demethylase dead Utx mutant gene knock-in. No UTX/PTIP/RbBP5 complex was precipitated from Utx-knockout ESCs (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Six4 with UTX (KDM6A) using nuclear extract from murine C2C12 myoblasts 24 hrs post myogenic differentiation (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).
Western Blotting Analysis: A representative lot detected UTX4 in myoblast whole cell lysates and embryonic stem cell (ESC) nuclear extracts from wild-type or Utx(KI/KI) mice harboring homozygous demethylase dead Utx mutant gene knock-in, as well as in UTX and MLL4 immunoprecipitates. No UTX target band was detected in Utx-knockout ESCs (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Western Blotting Analysis: A representative lot detected UTX (KDM6A) in nuclear extracts from human K562 and murine C2C12 myoblasts (24 hrs post myogenic differentiation), as well as in Six4 immunoprecipitates. shRNA treatment prior to nuclear extract preparation diminished target band detection (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Qualità
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected UTX (KDM6A) in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected UTX (KDM6A) in 10 µg of HeLa nuclear extract.
Descrizione del bersaglio
~154 kDa observed. 154.2 kDa (human; UniProt O15550) and 154.4/157.5 kDa (mouse isoform 1/2; UniProt O70546) calculated. Uncharacterized bands may be observed in some lysate(s).
Stato fisico
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Altre note
Concentration: Please refer to lot specific datasheet.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Non trovi il prodotto giusto?
Prova il nostro Motore di ricerca dei prodotti.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Jaclyn Andricovich et al.
Cancer cell, 33(3), 512-526 (2018-03-14)
KDM6A, an X chromosome-encoded histone demethylase and member of the COMPASS-like complex, is frequently mutated in a broad spectrum of malignancies and contributes to oncogenesis with poorly characterized mechanisms. We found that KDM6A loss induced squamous-like, metastatic pancreatic cancer selectively
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.