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Documenti fondamentali

ABE1387

Sigma-Aldrich

Anti-Nuclear factor 1/C Antibody

serum, from rabbit

Sinonimo/i:

Nuclear factor 1 C-type, NF1-C, CCAAT-box-binding transcription factor, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/C, NF-I/C, NFI-C, TGGCA-binding protein

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

serum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

mouse, rat, human

tecniche

ChIP: suitable
electrophoretic mobility shift assay: suitable
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... NFIC(4782)

Descrizione generale

Through evolution, eukaryotic cells have incorporated numerous viral promoter elements and incorporated them as part of their own gene regulatory elements. Nuclear factor 1/C was originally recognized as a transcription factor that bound the origin of replication for adenovirus type 2. Nuclear factor 1/C belongs to the CTF/NF-I family of eukaryotic DNA replication and transcription factors and is important in regulating the expression of many cellular developmental systems such as that of tooth development as well as multiple TGF-beta1 signaling mediated pathways systems.

Specificità

Recognizes multiple isoforms of Nuclear factor 1/C

Immunogeno

Epitope: C-terminus
Recombinant protein corresponding to full length Human NFIC.

Applicazioni

Chromatin Immunoprecipitation Assay (ChIP): A representative lot of this antibody has been reported to work in ChIP. See Hebbar, P.B., and Archer, T.K. (2007), Vicent G.P. et al. (2010), and Brun, M., et al. (2013).

Electrophoretic Mobility Shift Assay (EMSA): A representative lot of this antibody has been reported to work in EMSA. See Johansson E.M., et al. (2003) and Kannius-Janson, M., et al. (2002).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
This Anti-Nuclear factor 1/C Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of Nuclear factor 1/C.

Qualità

Evaluated by Western Blotting in U251 cell lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected NFIC isoforms at ~45-55 kDa in a U251 cell lysate.

Descrizione del bersaglio

~45-55 kDa observed

Stato fisico

Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Pratibha B Hebbar et al.
The Journal of biological chemistry, 282(11), 8284-8291 (2006-12-26)
Accessing binding sites in DNA wrapped around histones in condensed chromatin is an obstacle that transcription factors must overcome to regulate gene expression. Here we demonstrate cooperativity between two transcription factors, the glucocorticoid receptor (GR) and nuclear factor 1 (NF1)
Marie Kannius-Janson et al.
The Journal of biological chemistry, 277(20), 17589-17596 (2002-03-06)
Members of the nuclear factor 1 (NF1) transcription factor family have been postulated to be involved in the regulation of milk genes. In this work we have been able to identify the splice variant NF1-C2 as an important member of
Eva M Johansson et al.
Oncogene, 22(38), 6061-6070 (2003-09-05)
The p53 tumor suppressor protein plays an important role in preventing cancer development by arresting or killing potential tumor cells. Downregulated p53 levels, or mutations within the p53 gene, leading to the loss of p53 activity, are found in many
Namrata Rastogi et al.
Leukemia, 37(2), 276-287 (2022-12-27)
Nuclear factor I-C (NFIC) belongs to a family of NFI transcription factors that binds to DNA through CAATT-boxes and are involved in cellular differentiation and stem cell maintenance. Here we show NFIC protein is significantly overexpressed in 69% of acute
Calcineurin regulates nuclear factor I dephosphorylation and activity in malignant glioma cell lines.
Brun, M; Glubrecht, DD; Baksh, S; Godbout, R
The Journal of Biological Chemistry null

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