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Documenti fondamentali

AB5020

Sigma-Aldrich

Anti-Glycine (Low Glutaraldehyde) Antibody

diluted serum, Chemicon®

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

diluted serum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

rat

Produttore/marchio commerciale

Chemicon®

tecniche

immunohistochemistry: suitable

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Specificità

Glycine. The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable cross-reactivity (<1:1000) against glycine in peptides or proteins. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine.

Immunogeno

Glycine-glutaraldehyde-BSA

Applicazioni

Detect Glycine (Low Glutaraldehyde) using this Anti-Glycine (Low Glutaraldehyde) Antibody validated for use in IH.
Immunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.5% - 2.5% glutaraldehyde for optimum detection.

*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative). The minimum glutaraldehyde concentration for AB5020 is 0.05%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.

Optimal working dilutions must be determined by the end user.

DILUTION: Prepare enough of the AB5020 for your days use by diluting 100X with 1% GSPBT.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Confezionamento

2000 assays

Stato fisico

IgG fraction in sterile 0.1M phosphate buffer. No preservative

Stoccaggio e stabilità

Maintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze this stock.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Deepa M Deshpande et al.
Annals of neurology, 60(1), 32-44 (2006-06-28)
We explored the potential of embryonic stem cell-derived motor neurons to functionally replace those cells destroyed in paralyzed adult rats. We administered a phosphodiesterase type 4 inhibitor and dibutyryl cyclic adenosine monophosphate to overcome myelin-mediated repulsion and provided glial cell-derived
Optogenetic perturbation of preBotzinger complex inhibitory neurons modulates respiratory pattern.
Sherman, D; Worrell, JW; Cui, Y; Feldman, JL
Nature Neuroscience null
Franz Weber et al.
Nature, 526(7573), 435-438 (2015-10-08)
Rapid eye movement (REM) sleep is a distinct brain state characterized by activated electroencephalogram and complete skeletal muscle paralysis, and is associated with vivid dreams. Transection studies by Jouvet first demonstrated that the brainstem is both necessary and sufficient for
Nadia Parmhans et al.
The Journal of comparative neurology, 526(4), 742-766 (2017-12-09)
We report the retinal expression pattern of Ret, a receptor tyrosine kinase for the glial derived neurotrophic factor (GDNF) family ligands (GFLs), during development and in the adult mouse. Ret is initially expressed in retinal ganglion cells (RGCs), followed by
Lisa Nivison-Smith et al.
PloS one, 8(3), e58406-e58406 (2013-03-22)
Lampreys are one of the two surviving groups of the agnathan (jawless) stages in vertebrate evolution and are thus ideal candidates for elucidating the evolution of visual systems. This study investigated the retinal amino acid neurochemistry of the southern hemisphere

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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