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Key Documents

AB3066

Sigma-Aldrich

Anti-Aquaporin 2 Antibody

Chemicon®, from rabbit

Sinonimo/i:

AQP2, AQP-CD, MCH-CD

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

rat

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... AQP2(359)

Specificità

Aquaporin 2 (AQP-CD/WCH-CD).

SPECIES REACTIVITY: The antibody has not yet been tested for cross-reactivity to other species. The 15 aa C-terminal peptide has 100% homology with mouse and 93% homology with human AQP2.

Immunogeno

A 15 aa C-terminal peptide from rat AQP2. This peptide is predicted to be cytoplasmic.

Applicazioni

Detect Aquaporin 2 using this Anti-Aquaporin 2 Antibody validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western Blotting: 1-10 μg/mL for affinity pure antibody using Chemiluminescence technique. An antibody made to the AQP2 peptide detected a major band at ~29 kDa in rat kidney cortex, and 35-47 kDa in inner and outer medulla (postranslational or glycosylation) (Nielsen et al. 1993; Deen et al. 1995). A similar pattern is seen in the human kidney (Sasaki, et al. 1994).

Immunohistochemistry: 2-10 μg/mL in paraformaldehyde fixed sections of tissues. AQP2 has been localized in apical membranes (Fushimi et al. 1993; Nielsen et al. 1993; Deen et al. 1995).

ELISA: 0.5-1.0 μg/mL using 1 μg/mL control peptide/well.

Optimal working dilutions must be determined by end user.

Stato fisico

Affinity purified immunoglobulin.Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide.

Stoccaggio e stabilità

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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