AB3066

Anti-Aquaporin 2 Antibody

Chemicon^®, from rabbit

• Sinonimo/i: AQP2, AQP-CD, MCH-CD
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: rat
• Produttore/marchio commerciale: Chemicon^®
• tecniche: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• N° accesso NCBI: NM_000486.3
• N° accesso UniProt: P41181
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... AQP2(359)

Descrizione

Specificità

Aquaporin 2 (AQP-CD/WCH-CD).

SPECIES REACTIVITY: The antibody has not yet been tested for cross-reactivity to other species. The 15 aa C-terminal peptide has 100% homology with mouse and 93% homology with human AQP2.

Immunogeno

A 15 aa C-terminal peptide from rat AQP2. This peptide is predicted to be cytoplasmic.

Applicazioni

Detect Aquaporin 2 using this Anti-Aquaporin 2 Antibody validated for use in ELISA, IH & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western Blotting: 1-10 μg/mL for affinity pure antibody using Chemiluminescence technique. An antibody made to the AQP2 peptide detected a major band at ~29 kDa in rat kidney cortex, and 35-47 kDa in inner and outer medulla (postranslational or glycosylation) (Nielsen et al. 1993; Deen et al. 1995). A similar pattern is seen in the human kidney (Sasaki, et al. 1994).

Immunohistochemistry: 2-10 μg/mL in paraformaldehyde fixed sections of tissues. AQP2 has been localized in apical membranes (Fushimi et al. 1993; Nielsen et al. 1993; Deen et al. 1995).

ELISA: 0.5-1.0 μg/mL using 1 μg/mL control peptide/well.

Optimal working dilutions must be determined by end user.

Stato fisico

Affinity purified immunoglobulin.Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide.

Stoccaggio e stabilità

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable