71259
Insect GeneJuice® Transfection Reagent
Proprietary liposome transfection reagent optimized for maximal transfection efficiency of Sf9 insect cells for baculovirus protein expression.
Sinonimo/i:
GeneJuice Transfection
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About This Item
Codice UNSPSC:
41106502
NACRES:
NA.54
Prodotti consigliati
Livello qualitativo
Stato
suspension
Produttore/marchio commerciale
Novagen®
Condizioni di stoccaggio
OK to freeze
tecniche
transfection: suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
Insect GeneJuice<TMSYMBOL></TMSYMBOL> Transfection Reagent is a proprietary liposome formulation optimized for maximal transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for cotransfection of transfer plasmids with linearized virus DNA for the production of recombinant baculoviruses. Insect GeneJuice is ideal for large-scale protein expression in suspension-culture transfections of Sf9 insect cells when using pIEx<TMSYMBOL></TMSYMBOL> and pBiEx<TMSYMBOL></TMSYMBOL> vectors, which contain the immediate early baculovirus promoter, IE1.
Insect GeneJuice Transfection Reagent is provided as a 2 mg/ml suspension in 20 mM MES, 150 mM NaCl, pH 6.2 buffer. One ml is sufficient for 12 or 125 transfections in 10-ml suspension culture flasks or 35-mm plates, respectively.
- When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 75,726 when other reagent used with Sf9, thus 1.7 fold difference.
- When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 11,952 when other reagent used with High Five, thus 11 fold difference.
- When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 31,897 when Insect Genejuice used with High Five, thus 4.1 fold difference.
Insect GeneJuice Transfection Reagent is provided as a 2 mg/ml suspension in 20 mM MES, 150 mM NaCl, pH 6.2 buffer. One ml is sufficient for 12 or 125 transfections in 10-ml suspension culture flasks or 35-mm plates, respectively.
Attenzione
Toxicity: Standard Handling (A)
Note legali
GeneJuice is a registered trademark of EMD Biosciences, Inc., Madison, WI USA
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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