524625
Phosphatase Inhibitor Cocktail II
liquid, for the inhibition of acid phosphatases, alkaline phosphatases and protein tyrosine phosphatases (PTP's). Suitable for use with cell lysates and tissue extracts.
Sinonimo/i:
Phosphatase Inhibitor, Phosphatase Inhibitor Solution (Set II)
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.77
Prodotti consigliati
product name
Phosphatase Inhibitor Cocktail Set II, A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts.
Livello qualitativo
Forma fisica
liquid
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents.
Applicazioni
Phosphatase Inhibitor Cocktail Set II has been used:
- in triple detergent buffer to lyse cultured cells to extract total protein for western blots
- as a component of lysis buffer to lyse tissue samples or cultured rat ventricular cardiomyocytes (VCMs) for western blot analysis of cardiac mitochondrial proteins
- as a component of lysis buffer to lyse cultured rat VCMs infected with Adv-COX7RP and Adv-shRNA COX7RP for immunoprecipitation of ryanodine receptors type 2 (RyR2)
- as a component in ice-cold RIPA buffer to homogenize/lyse retina tissues for protein extraction and western blotting
Azioni biochim/fisiol
Protein phosphorylation and dephosphorylation play essential roles in signal transduction processes that regulate various cellular functions including cell division, proliferation, and apoptosis. Protein kinases transfer phosphate groups to specific serine, threonine, or tyrosine residues, leading to a conformational change in the protein and affecting its interaction with ligands. Protein phosphatases reverse this process by removing the phosphate group and restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain the phosphorylation state of the proteins intact.
Caratteristiche e vantaggi
- Flexible: compatible with cell lysates and tissue extracts, including samples containing detergents.
- Economical: ready-to-use format minimizes wastage.
- Effective: one ml is adequate for approximately 100 ml extraction buffer.
- Complete: contains five phosphatase inhibitors targeting acid and alkaline phosphatases and protein tyrosine phosphatases (PTPs).
Componenti
Provided as a set of five 1 ml vials containing the following components:
- Imidazole (200 mM)
- Sodium Fluoride (100 mM)
- Sodium Molybdate (115 mM)
- Sodium Orthovanadate (100 mM)
- Sodium Tartrate Dihydrate (400 mM)
Attenzione
Toxicity: Standard Handling (A)
Altre note
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Codice della classe di stoccaggio
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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