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Key Documents

481001

Sigma-Aldrich

Anti-Neutrophil Elastase Rabbit pAb

liquid, Calbiochem®

Sinonimo/i:

Neutrophil elastase antibody, Rabbit monoclonal antibody

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

rabbit

Livello qualitativo

100

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

human

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

Isotipo

IgG

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... ELANE(1991)

Descrizione generale

Ammonium sulfate precipitated rabbit polyclonal antibody. Recognizes human neutrophil elastase.
Recognizes the neutrophil elastase protein.
This Anti-Neutrophil Elastase Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoelectrophoresis, Immunoprecipitation for the detection of Neutrophil Elastase.

Immunogeno

Human
human neutrophil elastase

Applicazioni

ELISA (≥ 1:1000)

Immunoblotting (see comments)

Immunoelectrophoresis (see comments)

Immunoprecipitation (see comments)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In PBS, pH 7.5.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Altre note

Single arc by immunoelectrophoresis against neutrophil granule extract and human neutrophil elastase. This antibody has also been reported to work for immunoblotting and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Shigeto Hamaguchi et al.
The Journal of international medical research, 41(1), 162-168 (2013-04-10)
Neutrophils are able to form 'neutrophil extracellular traps' (NETs), which they use to trap and kill pathogens such as bacteria and fungi at the foci of infection. This observational study investigated the presence of NETs in the blood from critically
Volker Brinkmann et al.
Frontiers in immunology, 7, 513-513 (2016-12-07)
The pathogenic potential of neutrophil extracellular traps (NETs) was recently described, and their detection in tissue could serve as a prognostic marker. NETs are delicate and filigree structures; hence good tissue preservation is essential for their detection. Indeed, analysis of
Elaine F Kenny et al.
eLife, 6 (2017-06-03)
Neutrophils release neutrophil extracellular traps (NETs) which ensnare pathogens and have pathogenic functions in diverse diseases. We examined the NETosis pathways induced by five stimuli; PMA, the calcium ionophore A23187, nigericin, Candida albicans and Group B Streptococcus. We studied NET
Jiram Torres-Ruiz et al.
Frontiers in immunology, 12, 689966-689966 (2021-09-28)
Most of the explanatory and prognostic models of COVID-19 lack of a comprehensive assessment of the wide COVID-19 spectrum of abnormalities. The aim of this study was to unveil novel biological features to explain COVID-19 severity and prognosis (death and
Dae-goon Yoo et al.
Journal of immunology (Baltimore, Md. : 1950), 192(10), 4728-4738 (2014-04-18)
Cystic fibrosis (CF) airways are characterized by bacterial infections, excess mucus production, and robust neutrophil recruitment. The main CF airway pathogen is Pseudomonas aeruginosa. Neutrophils are not capable of clearing the infection. Neutrophil primary granule components, myeloperoxidase (MPO) and human
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