475935

MT1-MMP, Catalytic Domain, Human, Recombinant, E. coli

• Sinonimo/i: MMP-14 Catalytic Domain
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Saggio: ≥90% (SDS-PAGE)
• Livello qualitativo: 100
• Forma fisica: liquid
• Attività specifica: ≥140 mU/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Note: 1 mU = 1 milliunit.

Recombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). Degrades fibrillar collagen Types I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13 resulting in enhanced proteolysis of the matrix for cell migration and proliferation.

Recombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). The catalytic domain is comprised of amino acid residues Tyr⁸⁹ to Gly²⁶⁵ of mature human MT1-MMP. Hydrolyzes fibrillar collagens I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13, resulting in enhanced proteolysis of the matrix for cell migration and proliferation.

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.

Ricostituzione

Following initial thaw, aliquot and freeze (-70°C).

Altre note

Amano, T., et al. 2005. Cell Res.15, 150.
Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
Sato, H., et al. 1994. Nature370, 61.
Knight, C.G., et al. 1992. REBS Lett.296, 263.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable