405210

ID-8

A cell-permeable indole compound that, when added to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF

• Sinonimo/i: ID-8, 1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol
• Formula empirica (notazione di Hill): C₁₆H₁₄N₂O₄
• Numero CAS: 147591-46-6
• Peso molecolare: 298.29
• Numero MDL: MFCD00593367
• Codice UNSPSC: 12352200
• NACRES: NA.51

Proprietà

• Livello qualitativo: 100
• Saggio: ≥95% (HPLC)
• Stato: powder
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: yellow
• Solubilità: DMSO: 50 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: [N+](=O)([O-])c1c2c([n](c1C)c3ccc(cc3)OC)cc(cc2)O
• InChI: 1S/C16H14N2O4/c1-10-16(18(20)21)14-8-5-12(19)9-15(14)17(10)11-3-6-13(22-2)7-4-11/h3-9,19H,1-2H3; VVZNWYXIOADGSW-UHFFFAOYSA-N

Descrizione

Descrizione generale

A cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Standard Handling (A)

Altre note

Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem.72, 1242.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3