X1875

Xanthine Oxidase from bovine milk

Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein

• Sinonimo/i: XOD, Xanthine:oxygen oxidoreductase
• Numero CAS: 9002-17-9
• Classificazione EC (Enzyme Commission): 1.17.3.2.
• Numero CE: 232-657-6
• Numero MDL: MFCD00082145
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Tipo: Grade I
• Forma fisica: ammonium sulfate suspension
• Attività specifica: ≥0.4 units/mg protein
• Attività estranea: uricase ≤0.5%
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.

Formerly E.C. 1.1.3.22

Applicazioni

Xanthine Oxidase from bovine milk has been used:

• in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay
• in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity
• as a standard to determine XO activity
• as a standard to test the synergistic effect of docosahexaenoic acid (DHA)

Azioni biochim/fisiol

Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.

Definizione di unità

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Stato fisico

Suspension in 2.3 M (NH₄)₂SO₄ containing 1 mM sodium salicylate

Risultati analitici

Protein determined by biuret

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P304 + P340 + P312 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)