361527

PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

liquid, clone 2D3, Calbiochem^®

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 2D3, monoclonal
• Stato: liquid
• contiene: ≤0.1% sodium azide as preservative (antibody only)
• Reattività contro le specie: mouse, human
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG1
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pSer9)
• Informazioni sul gene: human ... GSK3B(2932) ; mouse ... Gsk3B(56637)

Descrizione

Descrizione generale

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).

Immunogeno

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β

Applicazioni

ELISA (0.05 µg/ml)

Immunoblotting (0.5 µg/ml)

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.

Ricostituzione

Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.

Risultati analitici

Positive Control
Pervanadate-treated HepG2 (included)

Altre note

For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable