G7914

Anti-Glycogen Synthase Kinase-3β (GSK-3β) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Numero MDL: MFCD01633410
• Codice UNSPSC: 12352203
• NACRES: NA.44

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen 46 kDa
• Reattività contro le specie: human, rat
• tecniche: microarray: suitable; western blot: 1:2,000 using rat brain extract
• N° accesso UniProt: P49841
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GSK3B(2932) ; rat ... Gsk3b(84027)

Descrizione

Descrizione generale

Anti-Glycogen Synthase Kinase-3β (GSK-3β) is developed in rabbit using a synthetic peptide (KGDRGQTNNAASASASNST) corresponding to the C-terminal region of human GSK-3β conjugated to KLH as immunogen. Glycogen Synthase Kinase-3β (GSK-3β) is present in cytoplasm, nucleus and mitochondria. GSK-3 exists as two closely related protein kinases, termed GSK-3α and GSK-3β. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids. It shows a wide range of tissue expression with the highest expression in brain.

Glycogen synthase kinase-3 β (GSK3β) is a 47kD protein that belongs to serine/ threonine protein kinase family and is expressed ubiquitously as a constitutively active kinase. It has a pivotal role in regulating the degradation of β-catenin by the tumor suppressor gene product APC. Anti-Glycogen Synthase Kinase-3β (GSK-3β) antibody can be used in microarray. Rabbit anti- GSK3β antibody reacts specifically with rat GSK3β but not with GSK3α.

Immunogeno

Synthetic peptide corresponding to amino acids 403-420 of human glycogen synthase kinase 3 β (GSK3β), coupled to KLH. It is identical in rat and highly conserved in frog and zebrafish GSK3β.

Applicazioni

Anti-Glycogen Synthase Kinase-3β (GSK-3β) antibody produced in rabbit has been used in immunoblotting assays for analysis of protein expression.

Azioni biochim/fisiol

Glycogen synthase kinase-3 (GSK-3) specifically phosphorylates glycogen synthase, a critical enzyme regulating glucose storage, and mediates insulin regulation of glycogen synthesis.(8) GSK-3β phosphorylates regulatory sites in the central region of APC. Binding of β-catenin to APC, marking β-catenin for destruction, is dependent on phosphorylation of APC by GSK-3β. Thus, GSK-3β may play a critical role in regulating β-catenin stability during development and tumor progression. It is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable