239803

Cyclopamine

from Veratrum californicum, ≥97% (HPLC), solid, Sonic Hedgehog signaling antagonist, Calbiochem^®

• Sinonimo/i: Cyclopamine, V. californicum
• Formula empirica (notazione di Hill): C₂₇H₄₁NO₂
• Numero CAS: 4449-51-8
• Peso molecolare: 411.62
• Numero MDL: MFCD09878269
• Codice UNSPSC: 12352116
• NACRES: NA.77

Proprietà

• product name: Cyclopamine, V. californicum, Cyclopamine, V. californicum, CAS 4449-51-8, is a cell-permeable steroidal alkaloid & cholesterol mimic that specifically antagonizes Sonic Hedgehog signaling through direct interaction with Smo.
• Livello qualitativo: 100
• Saggio: ≥97% (HPLC)
• Forma fisica: solid
• Potenza: 20 nM IC₅₀
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: off-white
• Solubilità: DMF: 10 mg/mL; DMSO: 4 mg/mL; ethanol: 5 mg/mL
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• InChI: 1S/C27H41NO2/c1-15-11-24-25(28-14-15)17(3)27(30-24)10-8-20-21-6-5-18-12-19(29)7-9-26(18,4)23(21)13-22(20)16(27)2/h5,15,17,19-21,23-25,28-29H,6-14H2,1-4H3/t15-,17+,19-,20-,21-,23-,24+,25-,26-,27-/m0/s1; QASFUMOKHFSJGL-LAFRSMQTSA-N

Descrizione

Descrizione generale

A cell-permeable steroidal alkaloid and cholesterol mimic that displays both teratogenic and antitumor activities. It disrupts cholesterol bio-synthesis and specifically antagonizes shh (Sonic Hedgehog) signaling pathway through direct interaction with Smo (smoothened), a distant relative of G-protein-coupled receptors. A valuable tool for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control. Also available as a 10 mM solution in Ethanol (Cat. No. 239806).

A cell-permeable, steroidal alkaloid and cholesterol mimic that displays both teratogenic and anti-tumor properties. Disrupts cholesterol biosynthesis and specifically antagonizes shh (Sonic Hedgehog) signaling through direct interaction with Smo (smoothened), a protein that is related to G-protein-coupled receptors. Useful for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control.

Cyclopamine, V. californicum, CAS 4449-51-8, is a cell-permeable steroidal alkaloid & cholesterol mimic that specifically antagonizes Sonic Hedgehog signaling through direct interaction with Smo.

Azioni biochim/fisiol

Cell permeable: yes

Primary Target
Hh signaling in Shh-light2 assay

Product does not compete with ATP.

Attenzione

Toxicity: Carcinogenic / Teratogenic (D)

Nota sulla preparazione

Ethanol stock solutions may require warming to achieve complete solubilization.

Ricostituzione

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Altre note

Berman, D.M., et al. 2003. Nature,425, 846.
Thayer, S.P., et al. 2003. Nature,425, 851.
Watkins, D.N., et al. 2003. Nature422, 313.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.
Berman, D.M., et al. 2002. Science297, 1559.
Taipale, J., et al. 2000. Nature406, 1005.
Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA95, 13036.
Cooper, M.K., et al. 1998. Science280, 1603.
Incardona, J.P., et al. 1998. Development125, 3553.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS06,GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H301,H360D
• Consigli di prudenza: P202 - P264 - P280 - P301 + P310 - P405 - P501
• Classi di pericolo: Acute Tox. 3 Oral - Repr. 1B
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable