1.50442

SeQuant^™ ZIC^®-HILIC 100Å (3.5 µm) HPLC Columns

L × I.D. 150 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

• Sinonimo/i: Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
• Codice UNSPSC: 41115709

Proprietà

• product name: SeQuant^™ ZIC-HILIC^™ 3.5µm,100Å 150 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
• Materiali: PEEK-lined stainless steel column
• Livello qualitativo: 100
• agenzia: suitable for USP L114
• Nome Commerciale: SeQuant^™
• Caratteristiche: endcapped: no
• Parametri: 550 bar pressure; 70 °C max. temp.
• tecniche: HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• Lungh. × diam. int. colonna: 15 cm × 2.1 mm
• Matrice: fully porous particle
• Gruppo funzionale matrice: ZIC-HILIC bonding phase
• Dimensione particelle: 3.5 μm
• Dimensione pori: 100 Å pore size
• pH di lavoro: 2-7.5
• Tecnica di separazione: hydrophilic interaction (HILIC)
• Temperatura di conservazione: no temp limit

Descrizione

Descrizione generale

SeQuant^™ ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.The column dimension of 150 mm length x 2.1 mm ID is the optimal choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^™ ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Applicazioni

• Synthesis of weak cation exchange/C(18) bifunctional magnetic polymers: Demonstrates their application in the pretreatment and determination of glufosinate and its metabolites in plasma, enhancing bioanalytical applications (Sun D, Xu Z, 2024).
• Highly sensitive measurement of underivatized phosphoenolpyruvate: Utilizes EDTA-facilitated HILIC-MS for accurate detection in biological samples, critical for metabolic studies (Shen D, Wang N, 2024).
• Comparison of RP, HILIC, and PGC columns for toxicometabolomics: Evaluates different chromatographic techniques for comprehensive metabolic profiling in biomedicine (Hemmer S, Meyer MR, 2024).

Risultati analitici

Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40

Note legali

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany