1.03858
GranuCult® plus Eosin Methylene Blue Agar
acc. HOLT-HARRIS and TEAGUE; For the isolation and differentiation of E. coli and other gram-negative bacteria
Sinonimo/i:
EMB agar, Holt-Harris & Teague (HHT) EMB agar, Eosin Methylene Blue Lactose Sucrose agar
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About This Item
Codice UNSPSC:
41106213
NACRES:
NA.74
Prodotti consigliati
Livello qualitativo
Forma fisica
powder
Condizioni di stoccaggio
dry media
tecniche
microbiological culture: suitable
pH
7.1±0.2 (25 °C)
applicazioni
food and beverages
pharmaceutical
Temperatura di conservazione
15-25°C
Compatibilità
selective for coliforms: Citrobacter spp.
selective for enterobacteriaceae (Genus: Proteus Vulgaris)
Descrizione generale
GranuCult® plus EMB Agar acc. HOLT-HARRIS and TEAGUE is a low selective and differential medium containing lactose and sucrose and two indicator dyes, Eosin and Methylene Blue. The use of these two indicators allows differentiation between colonies of lactose and/or sucrose fermenting and non-fermenting organisms. Sucrose is included since certain members of the coliform group of microorganism ferment sucrose more readily than lactose. This formulation does not discriminate between carbohydrate utilization (lactose or sucrose).
The peptones provide nitrogen, minerals and amino acids, lactose and sucrose provide the carbon sources. Di-potassium phosphate acts as a buffer whilst Eosin yellowish (Eosin Y) and methylene blue serve as differential indicators and inhibitors. Agar is the solidifying agent.
Coliforms produce blue-black colonies due to the incorporation of an eosin-methylene blue dye complex when the pH drops down. Colonies of Escherichia coli may show a characteristic green metallic luster due to the rapid fermentation of lactose. Lactose- and sucrose-non fermenting bacteria (like most Salmonella and Shigella) will form colourless or transparent amber-coloured colonies, but some strains of Salmonella and Shigella may not grow on this culture medium. Some Gram-positive bacteria and yeasts may grow on this culture medium and usually form pinpoint colonies.
The peptones provide nitrogen, minerals and amino acids, lactose and sucrose provide the carbon sources. Di-potassium phosphate acts as a buffer whilst Eosin yellowish (Eosin Y) and methylene blue serve as differential indicators and inhibitors. Agar is the solidifying agent.
Coliforms produce blue-black colonies due to the incorporation of an eosin-methylene blue dye complex when the pH drops down. Colonies of Escherichia coli may show a characteristic green metallic luster due to the rapid fermentation of lactose. Lactose- and sucrose-non fermenting bacteria (like most Salmonella and Shigella) will form colourless or transparent amber-coloured colonies, but some strains of Salmonella and Shigella may not grow on this culture medium. Some Gram-positive bacteria and yeasts may grow on this culture medium and usually form pinpoint colonies.
Applicazioni
GranuCult® plus Eosin Methylene Blue Agar is a low selective, differential agar used to identify coliforms especially E. coli. It differentiates lactose fermenters from non-lactose fermenters.It is often the media used when testing the potability of water via the MPN method
Caratteristiche e vantaggi
- Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
- Excellent wettability, solubility, and free flowing properties
- Convenient, with minimal component separation and clumping, even under warm or humid conditions
- High batch-to- batch reproducibility
- Prolonged shelf life of up to five years
- High number of test strains exceeding all regulatory demands
- Granulation technology allowing many supplements to be included, with no need to add these separately
Nota a piè di pagina
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Note legali
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
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