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Merck
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Key Documents

07-810

Sigma-Aldrich

Anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) Antibody

Upstate®, from rabbit

Sinonimo/i:

c-Met, cmet, HGFR, RCCP2, met proto-oncogene (hepatocyte growth factor receptor)

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

mouse, human

Reattività contro le specie (prevista in base all’omologia)

rat (based on 100% sequence homology)

Produttore/marchio commerciale

Upstate®

tecniche

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

Isotipo

IgG

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

phosphorylation (pTyr1230/pTyr1234/pTyr1235)

Informazioni sul gene

human ... MET(4233)
mouse ... Met(17295)
rat ... Met(24553)

Specificità

Recognizes Met phosphorylated on both Tyr1234/Tyr1235 and Tyr1230/Tyr1234/Tyr1235.

Immunogeno

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-c-Met (Tyr1230/Tyr1234/Tyr1235)

Applicazioni

Anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) Antibody is an antibody against phospho-Met (Tyr1230/Tyr1234/Tyr1235) for use in IC, IF & WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-Met (Tyr1230/Tyr1234/Tyr1235) in RIPA lysate of HGF treated 293T cells.

Qualità

Routinely evaluated by immunoblot.

Descrizione del bersaglio

155 kDa

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Federica Chiara et al.
The Journal of biological chemistry, 278(31), 29352-29358 (2003-05-15)
The met oncogene, encoding the high affinity hepatocyte growth factor receptor, is the only known gene inherited in human cancer that is invariably associated with somatic duplication of the mutant locus. Intriguingly, mutated Met requires ligand stimulation in order to
P Longati et al.
Oncogene, 9(1), 49-57 (1994-01-01)
The tyrosine kinase encoded by the MET proto-oncogene (p190MET) is the receptor for Hepatocyte Growth Factor/Scatter Factor (HGF/SF). Previous work has shown that autophosphorylation of p190MET enhances its enzymatic activity and that the major phosphorylation site is Tyr1235, located in
c-Met modulates RPE migratory response to laser-induced retinal injury.
Kasaoka, M; Ma, J; Lashkari, K
Testing null
A selective c-met inhibitor blocks an autocrine hepatocyte growth factor growth loop in ANBL-6 cells and prevents migration and adhesion of myeloma cells
Hov, H?kon, et al
Clinical cancer research : an official journal of the American Association for Cancer Research, 10, 6686-6694 (2004)
Helena L Palka et al.
The Journal of biological chemistry, 278(8), 5728-5735 (2002-12-12)
The receptor protein-tyrosine phosphatase (PTP) DEP-1 (CD148/PTP-eta) has been implicated in the regulation of cell growth, differentiation, and transformation, and most recently has been identified as a potential tumor suppressor gene mutated in colon, lung, and breast cancers. We have

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