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Key Documents

04-1550

Sigma-Aldrich

Anti-SC-35 Antibody, clone 1SC-4F11

ascites fluid, clone 1SC-4F11, from mouse

Sinonimo/i:

Protein PR264, Splicing component, 35 kDa, Splicing factor SC35, splicing factor, arginine/serine-rich 2

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

1SC-4F11, monoclonal

Reattività contro le specie

human, mouse, rat

tecniche

immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... SRSF2(6427)

Descrizione generale

SC-35 is also known as Splicing factor SC35 and Splicing factor, arginine/serine-rich 2. This protein is encoded by the SFRS2 gene and is a member of the splicing factor SR family. SC-35 is involved in pre-mRNA splicing and is found in bodies in the nucleus that are highly enriched in poly(A) RNA. These nuclear bodies are referred to as speckles, SC 35 domains, or splicing factor compartments (SFCs). The purpose of these bodies is still widely disputed. Speckles correspond largely to, if not entirely to, ultra structures called inter-chromatin granule clusters (IGCs). SC3-35 is required for pre-mRNA splicing events and is highly involved in spliceosomal interaction and assembly. SC-35 is necessary for assembly of the ATP-dependent splicing complex. It is also necessary for the ATP-dependent communication between pre-mRNA and U1 and U2 snRNPs.

Specificità

This antibody recognizes SC35.

Immunogeno

Epitope: Unknown
Ovalbumin-conjugated linear peptide corresponding to human SC35.

Applicazioni

Anti-SC-35 Antibody, clone 1SC-4F11 is a Mouse Monoclonal Antibody for detection of SC-35 also known as Protein PR264, Splicing factor SC35 & has been validated in WB, ICC.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SC35 in NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Qualità

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Sc35 on 10 µg of A431 cell lysate.

Descrizione del bersaglio

~ 35 kDa

Stato fisico

Unpurified
Unpurified mouse monoclonal IgG1κ preservative-free ascites.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
A431 cell lysate

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Hanna Kędzierska et al.
International journal of molecular sciences, 17(10) (2016-10-01)
Serine and arginine rich splicing factor 2(SRSF2) belongs to the serine/arginine (SR)-rich family of proteins that regulate alternative splicing. Previous studies suggested that SRSF2 can contribute to carcinogenic processes. Clear cell renal cell carcinoma (ccRCC) is the most common subtype
E Werwein et al.
Cell death & disease, 4, e511-e511 (2013-03-02)
B-Myb is a highly conserved member of the Myb transcription factor family that has essential roles in cell-cycle progression. Recent work has suggested that B-Myb is also involved in the cellular DNA-damage response. Here, we have investigated the fate of
Anastasia Selyutina et al.
iScience, 25(1), 103593-103593 (2022-01-11)
The HIV-1 capsid is the target for the antiviral drugs GS-CA1 and Lenacapavir (GS-6207). We investigated the mechanism by which GS-CA1 and GS-6207 inhibit HIV-1 infection. HIV-1 inhibition by GS-CA1 did not require CPSF6 in CD4+ T cells. Contrary to PF74
Ferdous Kadri et al.
The Journal of biological chemistry, 290(52), 30931-30946 (2015-11-05)
The HIV-1 transactivator protein Tat is implicated in the neuronal damage that contributes to neurocognitive impairment affecting people living with HIV/AIDS. Aberrant splicing of TAU exon 10 results in tauopathies characterized by alterations in the proportion of TAU isoforms containing
Jana Kralovicova et al.
Nucleic acids research, 42(12), 8161-8173 (2014-06-20)
Splice-switching oligonucleotides (SSOs) have been widely used to inhibit exon usage but antisense strategies that promote removal of entire introns to increase splicing-mediated gene expression have not been developed. Here we show reduction of INS intron 1 retention by SSOs

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