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Key Documents

GE17-0948-01

Blue Sepharose 6 Fast Flow

Cytiva 17-0948-01, pack of 50 mL

Sinonimo/i:

Blue Sepharose Fast Flow

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

ligand

Cibacron Blue

Confezionamento

pack of 50 mL

Produttore/marchio commerciale

Cytiva 17-0948-01

Condizioni di stoccaggio

(20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)

Matrice

6% cross-linked agarose

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning in place

3-13

working range

4-12

Compatibilità

suitable for bioprocess medium

Temperatura di conservazione

2-8°C

Descrizione generale

Blue Sepharose 6 Fast Flow is Cibacron Blue 3G coupled to Sepharose 6 Fast Flow. This medium is well established as adsorbent for albumin and interferon at both laboratory and process scale.

Blue Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Blue Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.
The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

Blue Sepharose 6 Fast Flow has been developed and tested in co-operation with leading large scale manufacturers and is used in routine commercial production.

Blue Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Blue Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caratteristiche e vantaggi

  • Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

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Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolli

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

This page shows how to remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from GE Healthcare.

Performing a Purification of NAD+-dependent dehydrogenases and ATP-dependent kinases with 5’ AMP Sepharose 4B, HiTrap Blue HP and Blue Sepharose 6 Fast Flow

This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from GE Healthcare.

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