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Key Documents

901757

Sigma-Aldrich

Low endotoxin gelatin from porcine skin

gel strength 100 (Bloom), <10 EU/g

Sinonimo/i:

beMatrix™ gelatin LS-W

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About This Item

Numero CAS:
9000-70-8
Codice UNSPSC:
41116130
NACRES:
NA.23

Forma fisica

powder

Impurezze

<10 EU/g Endotoxin
<10 EU/g
<300 g Total viable aerobic count

Perdita

<15% loss on drying

Colore

white to pale yellow

pH

5.0-6.5

Viscosità

1.8-2.8 mPa.s

Resistenza del gel

80-120 (bloom)

Anioni in tracce

sulfite (SO32-): <1.5 mg/ 20 g

Cationi in tracce

As: <1 ppm
Hg: <0.1 ppm
heavy metals: <20 ppm

Temperatura di conservazione

2-8°C

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Applicazioni

Gelatin has received significant attention in the biomedical field due to its inherent bioactivity. Low endotoxin gelatin from porcine skin, gel strength 100 bloom is a low bloom (low molecular weight) gelatin featuring less than 10 endotoxin units (EU) per gram. Endotoxins primarily consist of lipopolysaccharides from the outer membrane of gram-negative bacteria. Once in the bloodstream, endotoxins are capable of eliciting a strong immune response, potentially leading to fever, shock, a drop in blood pressure, and even potentially death. In addition to this immune response, several reports suggest endotoxins may also damage various types of cells. To avoid adverse effects, raw materials should contain the lowest level of endotoxins possible when used in biomedical or regenerative medicine research applications. This low endotoxin gelatin is derived from alkaline-treated porcine skin and has a variety of potential applications in the fields of regenerative medicine, medical devices, and tissue engineering research.

Note legali

beMatrix is a trademark of Nitta Gelatin

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
MKCH0151

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K W Brunson et al.
Journal of supramolecular structure, 9(2), 231-242 (1978-01-01)
Chinese hamster ovary (CHO . K1 . PRO) cell growth was inhibited by addition of a gram-negative bacterial lipopolysaccharide (LPS) to the cell culture medium. Growth inhibition began after three or four days of incubation, was dose-dependent up to a
T Kirikae et al.
International journal of immunopharmacology, 19(5), 255-262 (1997-05-01)
Trace amounts of endotoxin (lipopolysaccharide: LPS) are assumed to contaminate commercially available fetal bovine serum (FBS) for tissue or cell culture during the manufacturing process. We examined how cultured cells were affected by the endotoxin and how much endotoxin was
J G Brock-Utne et al.
Anaesthesia and intensive care, 17(1), 49-55 (1989-02-01)
Endotoxins (lipopolysaccharides, LPS) are potent bacterial poisons always present within the intestines in considerable amounts. Several pathophysiological conditions such as hypovolaemia, hypoxia, intestinal ischaemia, burns and radiation lead to a breakdown in the barrier and depending upon the extent of
Shoko Yamamoto et al.
Journal of materials science. Materials in medicine, 28(3), 48-48 (2017-02-09)
Calcium phosphate cements (CPCs), consisting of a mixture of calcium phosphate powders and setting liquid, have been widely used in orthopedic applications. One of the drawbacks of CPCs is their poor resorbability in the living body, which hinders substitution with
E J Ziegler et al.
The New England journal of medicine, 307(20), 1225-1230 (1982-11-11)
In an effort to decrease deaths from gram-negative bacteremia and endotoxin shock, we treated bacteremic patients with human antiserum to endotoxin (lipopolysaccharide) core. Antiserum was prepared by vaccinating healthy men with heat-killed Escherichia coli J5; this mutant lacks lipopolysaccharide oligosaccharide
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