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335975

Sigma-Aldrich

(+)-1-(9-Fluorenyl)ethyl chloroformate solution

18 mM in acetone, for chiral derivatization

Sinonimo/i:

(+)-FLEC solution

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About This Item

Formula empirica (notazione di Hill):
C16H13ClO2
Numero CAS:
Peso molecolare:
272.73
Numero MDL:
Codice UNSPSC:
12352005
ID PubChem:
NACRES:
NA.22

Grado

for chiral derivatization

Livello qualitativo

Densità del vapore

2 (vs air)

Tensione di vapore

180 mmHg ( 20 °C)

Forma fisica

liquid

Concentrazione

18 mM in acetone

Indice di rifrazione

n20/D 1.3602

Densità

0.79 g/mL at 25 °C

Gruppo funzionale

chloro

Temperatura di conservazione

2-8°C

InChI

1S/C16H13ClO2/c1-10(19-16(17)18)15-13-8-4-2-6-11(13)12-7-3-5-9-14(12)15/h2-10,15H,1H3
SFRVOKMRHPQYGE-UHFFFAOYSA-N

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Descrizione generale

(+)-1-(9-Fluorenyl)ethyl chloroformate is a highly fluorescent compound1 commonly used as a chiral derivatizing agent for the separation of racemates prior to reversed-phase HPLC analysis.

Applicazioni

  • Chiral analysis of β-methylamino alanine (BMAA) enantiomers: Details the use of (+)-1-(9-fluorenyl)-ethyl chloroformate (FLEC) for derivatization followed by LC-MS/MS analysis, improving the understanding of amino acids′ stereochemistry (Zurita et al., 2019).
  • Enantioselective micellar electrokinetic chromatography of dl‐amino acids: Utilizes (+)-1-(9-fluorenyl)ethyl chloroformate derivatization combined with UV-induced fluorescence detection to analyze amino acids, enhancing analytical methodologies (Prior et al., 2018).

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Dam. 1 - Flam. Liq. 2 - STOT SE 3

Organi bersaglio

Central nervous system

Rischi supp

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

1.4 °F

Punto d’infiammabilità (°C)

-17 °C


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D-amino acids (AA) analysis is becoming more and more relevant for metabolomics, therefore new analytical tools need to be developed. A common approach to achieve AA enantioseparation is chiral derivatization. Among the chiral derivatization reagents, (+) or (-)-1-(9-fluorenyl) ethyl chloroformate
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In the context of bioanalytical method development, process automatization is nowadays a necessity in order to save time, improve method reliability and reduce costs. For the first time, a fully automatized micellar electrokinetic chromatography-mass spectrometry (MEKC-MS) method with in-capillary derivatization

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