MAK567

Pyruvate Dehydrogenase Activity Assay Kit

sufficient for 100 colorimetric tests

• Synonyme(s) : PDH Activity Assay Kit
• Code UNSPSC : 41116104

Propriétés

• Utilisation: sufficient for 100 colorimetric tests
• Niveau de qualité: 100
• Méthode de détection: colorimetric
• Température de stockage: −20°C
• Informations sur le gène: human ... PDHA1(5160) , PDHA2(5161 ) , PDHB(5162) , PDHX(8050) ; mouse ... PDHA1(18597) , PDHA2(18598) , PDHB(68263) , PDHX(27402) ; rat ... PDHA1(29554) , PDHA2(117098) , PDHB(289950) , PDHX(311254)

Description

Description générale

Pyruvate Dehydrogenase (PDH) is a mitochondrial enzyme which has an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH (E1) forms a well-characterized enzyme complex (PDC). In the presence of NAD⁺ and CoA, PDH catalyzes the conversion of pyruvate into Acetyl-CoA and CO₂. Thus, PDH links the glycolysis metabolic pathway (cytoplasm) to the citric acid cycle (mitochondria). The Pyruvate Dehydrogenase Activity Assay Kit provides a sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various samples, based on a colorimetric method. In the assay, PDH activity changes a colorless developer to a colored product. The amount of the colorimetric (450 nm) product is proportional to the enzymatic activity present in the sample. The kit can detect PDH activity that is lower than 0.1 mU, in a variety of samples.

Application

Pyruvate Dehydrogenase (PDH) Activity Assay Kit has been used in the colorimetric measurement of PDH Activity in biological samples.

Caractéristiques et avantages

Sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various biological samples.
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

Adéquation

Suitable for measuring PDH Activity in adherent or suspension cells, from an animal or plant source.

Définition de l'unité

One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 µmole of NADH per minute at 37 °C at pH=7.5.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H341,H351
• Conseils de prudence: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Carc. 2 - Eye Irrit. 2 - Muta. 2 - Resp. Sens. 1 - Skin Irrit. 2
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3