C4129

α-Chymotrypsin from bovine pancreas

Type II, lyophilized powder, ≥40 units/mg protein

• Synonyme(s) : α-chymotrypsin A and B, alpha-chymotrypsin
• Numéro CAS: 9004-07-3
• Numéro de classification (Commission des enzymes): 3.4.21.1 (BRENDA, IUBMB)
• Numéro CE : 232-671-2
• Numéro MDL: MFCD00130481
• Code UNSPSC : 12352204
• eCl@ss : 42010112

Propriétés

• Type: Type II
• Niveau de qualité: 300
• Forme: lyophilized powder
• Activité spécifique: ≥40 units/mg protein
• Poids mol.: 25 kDa
• Composition: protein, ≥85%
• Couleur: white to off-white
• Numéro d'accès UniProt: P00767
• Application(s): diagnostic assay manufacturing
• Température de stockage: −20°C
• Informations sur le gène: cow ... CTRB1(618826)

Description

Application

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).

Actions biochimiques/physiologiques

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Notes préparatoires

Produced from 3× crystallized chymotrypsinogen

Remarque sur l'analyse

Protein determined by E1%/280

Autres remarques

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• Pictogrammes: GHS07,GHS08,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302 - H315 - H319 - H334 - H335 - H400
• Conseils de prudence: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves