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P4069
Phosphatase, alkalisch aus E. coli
buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
Synonym(e):
Orthophosphor-Monoesterphosphohydrolase (alkalisches Optimum)
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About This Item
Empfohlene Produkte
Biologische Quelle
Escherichia coli
Qualitätsniveau
Form
buffered aqueous glycerol solution
Spezifische Aktivität
20-50 units/mg protein (in glycine buffer)
Fremdaktivität
DNase, RNase, none detected
Lagertemp.
−20°C
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Anwendung
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.
Phosphatase, Alkaline from Escherichia coli has been used:
- in fluorometric assay to determine its enzyme activity
- in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
- in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity
Biochem./physiol. Wirkung
Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Vorsicht
Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.
Einheitendefinition
Eine Einheit hydrolysiert 1,0 μmol p-Nitrophenylphosphat je Min. bei einem pH-Wert von 10,4 und 37 °C.
Physikalische Form
Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl2, 0.5 mM ZnCl2, 50% (w/v) glycerol
Hinweis zur Analyse
Protein determined by biuret.
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Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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