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N8271

Sigma-Aldrich

α(2→3,6,8,9) Neuraminidase aus Arthrobacter ureafaciens

recombinant, expressed in E. coli, buffered aqueous solution

Synonym(e):

Neuraminidase aus Arthrobacter ureafaciens, Acyl-Neuraminyl-Hydrolase, Sialidase

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About This Item

CAS-Nummer:
9001-67-6
EC-Nummer:
3.2.1.18 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00131711
UNSPSC-Code:
12352204
NACRES:
NA.32

Rekombinant

expressed in E. coli

Qualitätsniveau

200

Form

buffered aqueous solution

Spezifische Aktivität

≥135 units/mg protein

Mol-Gew.

88 kDa
 95 kDa

Fremdaktivität

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

Versandbedingung

wet ice

Lagertemp.

2-8°C

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Verwandte Kategorien

Biochem./physiol. Wirkung

Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.

Verpackung

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Einheitendefinition

One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physikalische Form

Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.

Angaben zur Herstellung

Expressed in glycosidase-free hosts.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Michael Worobey et al.
Nature, 508(7495), 254-257 (2014-02-18)
Zoonotic infectious diseases such as influenza continue to pose a grave threat to human health. However, the factors that mediate the emergence of RNA viruses such as influenza A virus (IAV) are still incompletely understood. Phylogenetic inference is crucial to reconstructing
Nenad Macesic et al.
The Medical journal of Australia, 198(7), 370-372 (2013-04-16)
To review cases of nosocomial influenza and compare the epidemiology, clinical characteristics and outcomes with community-acquired cases. Prospective case series of adults hospitalised with influenza during April - November of 2010 and 2011 using a hospital-based sentinel surveillance system. A
Dominic Meusch et al.
Nature, 508(7494), 61-65 (2014-02-28)
Tripartite Tc toxin complexes of bacterial pathogens perforate the host membrane and translocate toxic enzymes into the host cell, including in humans. The underlying mechanism is complex but poorly understood. Here we report the first, to our knowledge, high-resolution structures
Audu J Natala et al.
Journal of medical entomology, 50(1), 85-93 (2013-02-23)
Amblyomma variegatum F. are obligate hematophagous ectoparasites of livestock that serve as the vectors of Ehrlichia ruminantium (formerly known as Cowdria ruminantium), the causative agent of heartwater disease. In the light of the fact that they are blood-feeding, their salivary
Johan Nordholm et al.
The Journal of biological chemistry, 288(15), 10652-10660 (2013-03-01)
Interactions that facilitate transmembrane domain (TMD) dimerization have been identified mainly using synthetic TMDs. Here, we investigated how inherent properties within natural TMDs modulate their interaction strength by exploiting the sequence variation in the nine neuraminidase subtypes (N1-N9) and the
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